I cloned 3HR and 5HR of p.falciparum on a plasmid and the next step is gRNA cloning. I did colony PCR for verification it is perfect but the thing is after taking the toothpeak of the colony in the petri dish should I also stream the leftover bacteria on the tip to a new plate of LB or not? is it necessary? cause the main colony already streamed once and for the second colony PCR do I need to streak it again? I will be thankful if you kindly share your experience.
Usually you pick part of a colony and use it for PCR and number it on the plate. If it has your correct plasmid you then can use the remainder of the colony to streak a new plate to maintain your strain or to inoculate a liquid culture for making glycerol stocks/further processing.
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