I'm going to insert DNA fragment into 'junk DNA' (non-transcribed, non-regulatory DNA region).
Although the target sites are junk DNA, it's intuitively obvious that the larger the size of inserted DNA fragment become, it's impact on neighboring genomic environment will be bigger as well.
Here, I need the rationale for the size of DNA fragment that allows minimal impact on genomic environment.
Does anyone know researches that I can cite for?
I don't think your assumption of intuitive obviousness is valid. I imagine the location is much more important than the size of the inserted DNA fragment. If you are inserting into a truly junk region then the size of the insert probably doesn't matter. If you are inserting near a gene then perhaps it might, depending upon whether or not you are separating upstream elements from a regulatory region.
In prokaryotes you can have large insertions or inversions that are much larger than single gene scale which have no impact on gene expression. In eukaryotes the genome organization of say a mouse is quite different than human yet most genes are similarly regulated.
Are there some locations where an insert might have an impact, of course there are. But I think in most cases not.
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