I am working on yeast transformation of a calcium channel. I performed twice transformation and no colony was observed after 3 days. The transformation protocol works well before. Is this gene toxic to yeast for the calcium channel activity? If so, How to achieve the transformation?
Hello,
You did not mention what kind of transformation you performed. Do you want to delete an yeast gene or introduce a gene from different species?
If it is the case that you want to delete an yeast gene, you can check the null mutant viability and phenotype from the SGD database. If it is an essential gene, you can think about conditional knockout.
Which method do you use for transformation? LiAc-ssDNA-PEG method works very well for any yeast transformation.
P.S. - Yeast transformation has nothing to do with colonialism. :D
Best wishes.
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