28 August 2024 2 10K Report

Hi I am making some rTaq by e.coli for our routine genotyping PCR. rTaq purified in house has a concentration unit of ug/ul instead of U/uL which is commonly used by commercial suppliers. We do not want to measure the unit activity by the standard protocol because we don't have those reagents, also knowing the activity unit precisely is not that necessary for genotyping. Just wondering, normally, for purified rTaq with good activity/purity, what's the typical activity unit per ug of the rTaq?

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