I hve brain striatum stored in freezer from which i have to isolate RNA. since the tissue is frozen how can i thaw it to cut the striatum into pieces?

06 June 2024 0 2K Report

Tissue needs to be cut to weigh the desired amount for Trizol homogenisation

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Can Quran independently considered as a Source of Seerah(Biography of the Prophet Muhammad)?

How can one interpret and understand the Quran as a primary source for the life and teachings of the Prophet Muhammad, without relying solely on secondary sources or traditional commentaries, by...

05 July 2024 5,713 9 View

I have lyophilized primers.Do i need any buffer or PCR graded water to dilute my primers ?

primers are to be used for RTPCR

03 June 2024 8,588 8 View

Need suggestions for a topic related to General Surgery on which I can do a meta-analysis on using RCTs preferably?

The topic can cover any area of this specialty.

29 May 2024 3,200 1 View

Can phosphorylated and biotinylated oligos be stored at -80, or should be stored at -20? What is better recommendation to increase the life?

Oligo length is 19bp

10 January 2024 4,299 1 View

How to establish a Mathematical Model on "Transfer of heat in human body organs"?

I'm looking for a mathematical model for transfer of heat in human body organs especially (eye, heart, brain and lungs). How is the behavior of heat transfer at normal and extreme levels

12 July 2023 4,888 2 View

Discovery studio error while plotting the 2D structure of protein-DNA interaction ?

Hello everyone, I am using the discovery studio for the visualization of the Protein-DNA interaction as the 2D plot but it shows some error the ligand fragment is not selected, how I fix this...

21 June 2023 9,436 4 View

If anyone knows a numerical calculation for the emission of lighter when CO2 = 5.4‰ alters into δ13C = 3.1‰ in inflowing river waters?

If anyone knows a numerical calculation for the emission of lighter when CO2= 5.4‰ alters into δ13C = 3.1‰ in inflowing river waters? Thanks In advance!

04 December 2022 3,893 2 View

Is there any way how to to interpret LCMS data as we are getting only mass spectrum of the compounds in case of plant extracts?

I have done LCMS of different extracts of my plant.I got only mass spectrum of the compounds which is dfficult to interpret as there are different compounds whose molecular weight is same.They...

23 November 2022 7,968 5 View

Why sometimes CHARMMGUI does not generate lig.prm parametric file?

i am doing MD simulation for protein, 6lu7. when i use charmmgui to generate parametric files lig.prm file is not generated.

07 March 2022 6,739 0 View

Kindly suggest the solution for namd2 error FATAL ERROR: UNABLE TO FIND ANGLE PARAMETERS FOR CG2O1 CG2R61 CG2R66 (ATOMS 3946 3944 3950)?

i have been doing the molecular dynamic simulation for 6lu7 protein and some ligands and constantly getting the namd2 error.

03 March 2022 9,117 2 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Strugglling with m6A dot blot any suugesstion ?

I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...

10 August 2024 8,539 5 View

RNA Extraction Using Hot Borate Method No Longer Working?

I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...

08 August 2024 9,882 2 View

Does Anyone have expertise in in vitro transcription and RNA pull down assay?

I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.

07 August 2024 6,622 1 View

Microtome tissue sectioning - How to prevent chatter of paraffin tissue specimen sections?

Hello everyone, I have recently started using the microtome device for sectioning of paraffin-embedded mice lung. While I had some success in sectioning and observing proper ribbons, some of my...

06 August 2024 666 4 View

E.coli contamination in human RNA seq data ?

Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...

06 August 2024 807 3 View

RNA later for the preservation of RNA in fecal samples at room temperature for one day (37°C)?

I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...

06 August 2024 1,367 3 View

Project/Experimental assistance ？?

Do experiments on behalf of Our Business: Laboratory animal rearing Animal modeling Experimental animal behavior detection, image ultrasonic detection, electrophysiology platform Organoid...

05 August 2024 5,829 1 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 501 2 View

How to Transform my handy-wavy explanation of why group I/II metals oxides in aqueous solution are basic into a rigorous argument?

I heard an explanation about something being a better proton acceptor or lone pair donor but that doesn't make sense. I couldn't explain in in terms of acid-base theory. The hand-waving way I saw...

03 August 2024 9,918 0 View