After extensive research, I found information only on the pGro7 plasmid. However, the inducing agents for pGro7 and pGro12 differ. I aim to use IPTG for protein expression, and literature indicates that pGro12 plasmids were constructed based on a 1998 reference or obtained through academic donations. Due to experimental requirements, I would greatly appreciate any information or resources you can share regarding pGro12. Thank you!
Hi Zhangrui,
the plasmids pGro7 and pGro12 are very siminlar. If you study the article from Nishihara at al. from 1998 carefully you will see that both plasmids are identical except for the resistence gene their harbour: pGro7 contains a chloramphenicol resistance while pGro12 contains a kanamycin resistence. chaperone expression is induced by adding L-arabinose for both plasmids. You can use both plasmids in co-expression experiments to optimize the expression of your protein of interest. There is a product manual from Takara for the "Chaperone Plasmid Set" with a vector map for pGro7 (you just need to replace the chloramphenicole resistance with the kanamycin resistance and you have pGro12). This manual also describes how to do the co-expression. I hope this helps
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