Mitochondrial membrane potential of plant cells is easily detectable using fluorescent microscope. If I want to quantify the changes in fluorescent intensity, how can I detect it?
A probable method of studying mitochondrial dysfunction by R123 or JC1 staining can be by the isolation of mitochondria, staining with these fluorescent dyes and quantification of fluorescence by spectrofluorimetry. But such a method requires a large amount of treated plant tissues and can be expensive and cumbersome. Usually most studies reply on confocal microscopy of the stained tissues followed by fluorescence quantification by Image J software. Another method is to isolate protoplasts from treated plant tissues, staining and spectrofluorimetric analysis. These links might provide some information.
http://onlinelibrary.wiley.com/doi/10.1111/j.1365-3040.2012.02506.x/pdf
http://download.springer.com/static/pdf/119/art%253A10.1186%252Fs40529-014-0067-1.pdf?originUrl=http%3A%2F%2Fas-botanicalstudies.springeropen.com%2Farticle%2F10.1186%2Fs40529-014-0067-1&token2=exp=1469780787~acl=%2Fstatic%2Fpdf%2F119%2Fart%25253A10.1186%25252Fs40529-014-0067-1.pdf*~hmac=4bc5a8a75b2e450474feba44d7fc3ad7eae3b23257eb65d55f1b018a1cebb429
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