What are the differences between BSM and BMGY medium?

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What is cause of decrease in effectiveness in fermentation scaling up process?

Hi, I am working on Pichia pastoris fermentation. I had runned on 7.5 litter scale, small scale. I have yet tried at 25 litter scale, big scale. The result shows I got failure in scaleup. You can...

03 April 2019 3,506 10 View

What make the different in protein expression of the same carbon source?

Hi, I am working on protein expression by Pichia pastoris. I focus on mixed feed to get higher result. I read many papers. Some of author said that glycerol is repression the AOX1 promoter, which...

04 May 2018 3,239 0 View

Why every time the target protein get out of the gel filtration column, it made overpressure?

Dear, I am doing in gel filtration. My protein is fgf2. In the first step of purification, I applied cation exchange chromatography, but it was not clear enough. I try to do the next step to get...

06 July 2017 9,311 5 View

Which perfect buffer should I use in Bradford assay with many protein sample?

Hi, I'm purifying a recombinant protein. It's secreted protein. After chromatography procedure, I have some samples of protein: - Fermentation sample (applied sample): the supernatant from BSM...

09 October 2016 1,230 12 View

How much samples can I apply to a cation exchange column?

Hi, I'm using Hitrap SP FF collumn (cation exchange collums) to purifiy PDGF. The collumn's details show binding capacity of some proteins: 70 mg Ribonuclease A/ml gel (Mw: 13 700 D) 50 mg human...

03 April 2015 2,420 6 View

Can FPLC destroy protein structure to many oligopeptides which can be not detected by SDS-PAGE electrophoresis?

I purify by FPLC by Hitrap SP FF column. I have 4 fractions and all of them were being collected. I take an SDS-PAGE electrophoresis, and get some things below: - Percentage of target protein in...

31 December 2014 2,150 10 View

How can I fix unknown lines in SDS-PAGE electrophoresis?

Dear, I take a SDS-PAGE electrophoresis gel. It appears some lines which I don't know what they are. The line connect all wells of gel and they are more than two. What's it and How to fix my...

11 December 2014 2,850 16 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

Microtome tissue sectioning - How to prevent chatter of paraffin tissue specimen sections?

Hello everyone, I have recently started using the microtome device for sectioning of paraffin-embedded mice lung. While I had some success in sectioning and observing proper ribbons, some of my...

06 August 2024 666 4 View

How much Yeast should we add to fermentation media?

I have prepared a liquid broth containing Yeast (S. Cerevisiae) that i need to add from it to fermentation media of lignocellulosic hydrolysate. I need to know based on what parameters do we add...

06 August 2024 662 1 View

How do soil microflora interact with plant roots and influence plant nutrition, health, and productivity?

06 August 2024 9,618 3 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 501 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

Good Day, can I ask for your help on the questionnaire that I want to adopt?

I am currently looking for a questionnaire that relates with my study which is The Effectiveness of Organizational Culture towards employee retention rates

03 August 2024 3,505 6 View

Can i quantify a nanoparticle encapsulated in cell culture medium by hplc?

Hello, if you made a transwell assay where you incubate the cells with a nanoparticle-encapsulated drug and considering that in the oposite compartment you'll have both the free and encapsulte...

03 August 2024 2,730 1 View