What is cause of decrease in effectiveness in fermentation scaling up process?

Hi, I am working on Pichia pastoris fermentation. I had runned on 7.5 litter scale, small scale. I have yet tried at 25 litter scale, big scale. The result shows I got failure in scaleup. You can...

03 April 2019 3,397 10 View

What make the different in protein expression of the same carbon source?

Hi, I am working on protein expression by Pichia pastoris. I focus on mixed feed to get higher result. I read many papers. Some of author said that glycerol is repression the AOX1 promoter, which...

04 May 2018 3,131 0 View

Why every time the target protein get out of the gel filtration column, it made overpressure?

Dear, I am doing in gel filtration. My protein is fgf2. In the first step of purification, I applied cation exchange chromatography, but it was not clear enough. I try to do the next step to get...

06 July 2017 9,231 5 View

Which perfect buffer should I use in Bradford assay with many protein sample?

09 October 2016 1,074 12 View

How much samples can I apply to a cation exchange column?

03 April 2015 2,326 6 View

Can FPLC destroy protein structure to many oligopeptides which can be not detected by SDS-PAGE electrophoresis?

31 December 2014 2,078 10 View

How can I fix unknown lines in SDS-PAGE electrophoresis?

Dear, I take a SDS-PAGE electrophoresis gel. It appears some lines which I don't know what they are. The line connect all wells of gel and they are more than two. What's it and How to fix my...

11 December 2014 2,760 16 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

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02 March 2021 3,060 3 View

What do you know about biotechnology art?

What do you know about biotechnology art?

02 March 2021 1,653 3 View

Which is the best place in the world for the plant biotechnology research?

I am searching for a good place for the Post Doc,

02 March 2021 4,053 3 View

Which bacteria are suitable for quorum sensing screening?

I want to find a quorum sensing inhibitor from plant extracts. Klebsiella pneumoniae, Moraxella catarrhalis, Staphylococcus aureus, and Streptococcus pneumoniae. I looked for these strains...

01 March 2021 7,675 3 View

Isolates of Hymenoscyphus fraxineus?

Dear Dr. Cai, my name is Simone Prospero and I work in the team of Phytopathology at the Swiss Federal Institute for Forest Snow and Landscape Research (WSL;...

01 March 2021 1,133 1 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View

How to remove fatty material from plant extract?

The resultant solid was partitioned three times with 250 ml of petroleum ether (40-60/c) to remove any fatty material and evaporated to dryness.

28 February 2021 9,464 2 View