Dear Researchers,
Is it possible for cotton seedlings (10 days old) to produce antioxidant enzymes in cotyledons and other parts under short duration (6h,12h and 24h) of salt and drought stress (stress treatment in distilled water supplemented with various concentrations of NaCl and PEG)? I came across so many articles dealing with leaf and other plant parts only not with cotyledons. I want to estimate antioxidants in whole seedlings after stress tretament. Kindly give your suggestions. Thanks in advance.
Hi, Prem,
I've estimated SOD activity in both the whole canola seedlings and roots, hypocotyls, and cotyledons separetely. May be it will be helpful for you.
There are several articles concerned SOD activity in the cotton cotyledons, for example
http://www.jmbfs.org/wp-content/uploads/2014/05/jmbfs_0612_sakhno.pdf
http://agris.fao.org/agris-search/search.do?recordID=CN19920083840
http://horizon.documentation.ird.fr/exl-doc/pleins_textes/pleins_textes_7/b_fdi_53-54/010016152.pdf
https://www.infona.pl/resource/bwmeta1.element.elsevier-3dc0f355-e854-37b7-9bc7-8575cfd1dcfb
I think it is OK to determine the enzymatic antioxidants in the cotton cotyledons under NaCl and drought stresses. I disagree with Rajesh on using NaCl to induce water deficit since ionic stress will be unavoidable factor. It is better to use PEG polymer of 8000 or more to induce water deficit. PEG 8000 or more decreased the growth medium water potential without entering the cells. It cannot pass through the interstices of the cell wall because PEG is bigger. You have to known this number 8000 is not a real molecular weight.
Greetings
Yes, it is possible to estimate antioxidant enzymes' in the cotton seedlings.
- Badges
- Science topic
- Similar topics
- Biological Science
- Botany
- Plant Biology
- Plant Biotechnology