I'm looking for a simple method for measuring interstellar- cytoplasmic pH of plant cells.
Please read your question prior to post it and reformulate it. Why?
- intercellular pH of what ??? plant cells, animal or human cells cultured cells e.g. monolayer celles like HeLa or 3 D tumor cells.....
Please search again in Google Scholar and NOT google and give your question in the google scholar mask.
- For interstellar-cytoplasmic pH, you need an UFO from another galaxy!!
- Define simple method and the kind of dye (see molecular probes on line).
here an abstract as help for a better formulation:
Abstract
The activity of most cellular processes is sensitive to pH. Cells therefore tightly control cytosol pH within narrow bounds. Measurement of cytosolic pH is of interest in studying many processes, including pH regulatory transport proteins. Key approaches that have been used to determine intracellular pH include pH-sensitive microelectrodes, nuclear magnetic resonance, and pH-sensitive fluorescent proteins. Here we review these approaches while providing details on the use of pH-sensitive fluorescent dyes to measure cytosolic pH.
JRG
Dear Dr. Grezes
thanks a lot for your kind and useful guides. I'll revise the question and research again.
king regards.
A.Shomali
At present, probably there is no 'simple' method in measuring intracellular pH of a cell.
Here is a related article entitled "Imaging Intracellular pH in Living Cells" for you. It is from this website: http://www.andor.com/learning-academy/imaging-intracellular-ph-in-living-cells-using-confocal-imaging-in-monitoring-cellular-ph
you can use a simple patch clamp method. Where you know the concentration/pH of buffer on one side, Perform the patch clamp and measure the change in pH after.
Sally Assman @ Penn State University specializes in patch clamp experiments at my school. You may want to look at some of her work.
Good Luck!
direct measurement for Guttation or extraction of water by pressure
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