Can someone share agro bacterium mediated transformation protocol for pCAMBIA 1305.1 in wheat please? I am using Bobwhite cultivar and C58C1 agro strain but the regeneration rate is very poor.
Yuan-Yeu Yau
Hi Ahsan,
The Triticum aestivum Spring “Bobwhite” is the most representative cultivar representing over 25% of the data reported of Agrobacterium-mediated transformation of wheat, becoming “the genotype model”
I attached an article for you. It summarizes the work of Agrobacterium- mediated transformation of wheat which researchers have done. It includes (1) genotypes of wheat used, (2) Agrobacterium strains, (3) Promoters, (4) Selection markers, and (5) Important factors affecting Agrobacterium transformation of wheat. See the yellow highlights.
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