I am currently trying to use green peach aphid(GPA), potato and potato viruses to conduct an experiment.
I have a Potato Leafroll Virus infected potato (Superior) and GPA colony grown from tobacco plant.
Although GPAs grow well in tobacco plant or Chinese cabbage, when I transfer them onto PLRV infected potato either using clipcage or directly onto the plant, they usually die soon.
For example, when I use clip cage to rear aptaerous adult aphids, they do well for 2 to 3 days but die afterwards. Also, when I just directly move them onto the plant, they either leave the plant and die on the ground.
I'm suspecting that years of growing GPA on tobacco might have reduced their fitness onto new potato plant. If that's so, is there any way to rear them on potato plants?
Also, could there be any other reason or any tips that you might be able to give me?
You may use a very fin brush when transferring aphids most likely the mouthpart destroyed then aphids died from hunger
Usually I start from newly emerged aphid put them for parafilm feeding on liquid sucrose syrup (and ELISA test to be sure of viral free), then next generation transmitted to healthy Chinese cabbage for aphid mass rearing. Then any experiment you easily may conducted by transferring aphid to potato infected or healthy plant under cages
If you need any details or help don't hesitate to ask. Several possibility Pls. find the attached files
http://www.nature.com/articles/srep21954
http://entnemdept.ufl.edu/creatures/veg/aphid/green_peach_aphid.htm
https://www.cabdirect.org/cabdirect/abstract/20143235109
https://www.ncbi.nlm.nih.gov/pubmed/5558295
https://research.cip.cgiar.org/confluence/display/SET/Virus+Protocol
https://www.researchgate.net/publication/233679523_Host_Preferences_of_the_Green_Peach_Aphid_Myzus_persicae_Hemiptera_Aphididae
http://www.researchjournal.co.in/upload/assignments/6_492-493.pdf
Hoping this will be helpful
Regards
Prof. Houda Kawas
Article Host Preferences of the Green Peach Aphid, Myzus persicae (H...
That was really helpful. Thank you so much for your detailed and kind answer!
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