During the multiplication stage in vitro, the shoots are sticking together is difficult to separate them later,what are the actions to avoid sticking?

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Why science doesn't work without curiosity?

Curiosity: It Helps Us Learn, But Why?

05 June 2017 8,893 99 View

What Is Greenhouse Gas?

Read 12 answers by scientists with 33 recommendations from their colleagues to the question asked by Ahmed Madi Al Mayahi on May 14, 2017

04 May 2017 3,062 12 View

Red blood cells their color red, of course , What is the color of White blood cells?

Read 1 answer by scientists with 1 recommendation from their colleagues to the question asked by Ahmed Madi Al Mayahi on Mar 18, 2017

02 March 2017 9,097 1 View

How do migrating organisms find their way?

Birds, butterflies, and whales make annual journeys of thousands of kilometers. They rely on any thing to find their way?

31 December 2016 5,440 11 View

How do you see your experience in registration in site of research gate?

Scientific research, questions, answers etc...

11 December 2016 7,065 6 View

Is Breakfast Really the Most Important Meal of the Day?

The idea that breakfast is the most important meal of the day has been ingrained in our minds. But is it true?

11 December 2016 804 11 View

Any of the plant parts are firstly affected by salinity ?

Cultured plants are exposed to many stresses, and salinity of the most important stresses that affect on plants in many regions of the world.

11 December 2016 8,522 5 View

What is the basis of variation in stress tolerance in plants?

Read 13 answers by scientists with 54 recommendations from their colleagues to the question asked by Ahmed Madi Al Mayahi on Dec 27, 2016

11 December 2016 8,770 13 View

How do plants make cell walls?

Read 5 answers by scientists with 3 recommendations from their colleagues to the question asked by Ahmed Madi Al Mayahi on Dec 26, 2016

11 December 2016 7,031 4 View

Show in some times poor growth of plants cultured in glass vessels due to vitrification? What are the reasons that lead to the emergence of that case?

Tissues , in vitro, White transparent

10 November 2016 381 2 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Microtome tissue sectioning - How to prevent chatter of paraffin tissue specimen sections?

Hello everyone, I have recently started using the microtome device for sectioning of paraffin-embedded mice lung. While I had some success in sectioning and observing proper ribbons, some of my...

06 August 2024 666 4 View

How do soil microflora interact with plant roots and influence plant nutrition, health, and productivity?

06 August 2024 9,618 3 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 501 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

Good Day, can I ask for your help on the questionnaire that I want to adopt?

I am currently looking for a questionnaire that relates with my study which is The Effectiveness of Organizational Culture towards employee retention rates

03 August 2024 3,505 6 View

Can i quantify a nanoparticle encapsulated in cell culture medium by hplc?

Hello, if you made a transwell assay where you incubate the cells with a nanoparticle-encapsulated drug and considering that in the oposite compartment you'll have both the free and encapsulte...

03 August 2024 2,730 1 View

How to Transform my handy-wavy explanation of why group I/II metals oxides in aqueous solution are basic into a rigorous argument?

I heard an explanation about something being a better proton acceptor or lone pair donor but that doesn't make sense. I couldn't explain in in terms of acid-base theory. The hand-waving way I saw...

03 August 2024 9,918 0 View