How to analyze qRT-PCR data by using delta delta Ct method when one sample has Ct value of GOI Zero?

I have qRT-PCR data of 4 transgenic line and 1 non-transgenic line. Non-trangenic line has GOI Ct value zero and HKG has Ct value because non-transgenic has no transgene/GOI, that's why no Ct...

05 June 2018 7,664 4 View

Why SmaI is not digesting the plasmid completely?

I have a construct having Restriction site SmaI+KpnI on both sides of gene. I want to lift the gene with SmaI and KpnI. SmaI linearized it, after it i purify with PCR kit and digest with KpnI....

08 September 2017 8,995 5 View

How to amplify a GC-rich gene from genome?

01 February 2017 5,841 6 View

which signal peptide should be used to target the (human or other) protein extracellular in transgenic plant? (secretory path like Bombyxin/insulin)

10 November 2016 3,072 1 View

What is the effect of high level GC content in a gene, on its expression level?

A gene (high level GC content 70 % as compared to other host gene )which is native and when express it under constitutive promoter in plant, not give high expression. what may be the cause?

09 October 2016 1,077 1 View

What points shoud be considered during codon optimization of glycosylated protein?

glycosylated protein. codon optimzation in such a way, that properly glycosylated and properly folded. what things should i ocnsider during codon optimzation

09 October 2016 606 3 View

WHY THE ph of CTAB should be 8?

thanks in advance

04 May 2015 4,565 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View