Why is urinary exosomes isolated at 200000 g whereas at 120000 g for cell derived exosomes as mentioned in several papers. Will not these two different speeds yield different vesicles of different size/characteristics. Can anyone help me with this?
I want to know more about Uranium ore deposits in world.
11 August 2024 6,718 0 View
I want to know more about diamond ore deposits in world.
11 August 2024 2,166 1 View
If Banks do not provide credit facility, what are the options available for FPOs and impact on producer’s income?
10 August 2024 8,197 5 View
We assume that the difference is huge and that it is not possible to compare the two spaces. The R^4 mathematical space considers time as an external controller and the space itself is immobile in...
10 August 2024 6,676 14 View
What are a “Farmers Producer Organization” (FPO) and its essential features?
10 August 2024 475 5 View
I used eye tracking to examine how participants from two different populations (A and B) react to an image. Participants in population A exhibit larger pupil sizes over time, but they also have...
10 August 2024 3,226 0 View
I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...
10 August 2024 8,537 5 View
How do interactions between the biosphere, the carbon cycle, and the water cycle impact global warming and interaction between the atmosphere and the hydrosphere?
09 August 2024 3,286 2 View
I have input a moment load in module load Abaqus, i put my moment load on the node surface (using reference point). I have define moment in history output and make a set for moment too. But the...
08 August 2024 4,829 4 View
How is energy cycled through the Earth's climate system and how do matter cycle and energy flow through the rock cycle?
08 August 2024 8,159 0 View
What should be the zeta potential of bovine milk exosomes
25 July 2024 7,376 5 View
Hello, I want to ask regarding exosome protein quantification method. Is it necessary to lyse exosomes before measuring their protein contents using BCA assay kit? Or can I just straight away...
23 July 2024 4,603 4 View
I placed mice in metabolic cages to collect urine samples for analysis. However, the majority of the samples have dried up. Is there any way to retrieve the urine in those tubes without...
14 July 2024 6,724 0 View
Hi, All, I am relatively new for exosome isolation. I used an izon column 70nm to isolate exosomes from differentiated neural cells, but the concentrations were pretty low after NTA analysis (I...
01 July 2024 2,556 0 View
I need an appropriate positive control for WB antibodies I am using to confirm the presence of exosomes in samples. The thing is I am having reproducibility issues, and I would like distinguish...
19 June 2024 4,209 3 View
What parameters are important in this process?
17 June 2024 4,463 0 View
I just found many protocols but not specific for bacterial growth
30 May 2024 6,304 2 View
Hello! I am planning on doing co-incubation experiments of neutrophils with exosomes and wanted to ask what concentration of exosomes, cell number and incubation time works with this cell...
29 May 2024 6,550 0 View
Hello! I'm hoping to run an ELISA on human urine samples to measure testosterone levels. We are hoping to use a kit from Neogen, which has been validated for serum/tissue/urine. The protocol...
28 May 2024 2,814 0 View
if we are doing non-invasive sensing in patient urine then require ethical permission or not??? If we have a consent form fill with the patient then it is sufficient or not???
24 May 2024 955 0 View