How to assess whether alternative splicing affects protein translation?

More Zhou Shumin's questions See All

Is there any method that we can electrochemically oxidize a powder?

I am working with a material which is sensible to water and oxygen, I would like to oxidize this powder either chemical or electromagnetically. Does anyone knows a method to electrochemically...

09 February 2021 2,944 3 View

How to make sure each cavity modes with same E-field distribution in COMSOL simulation?

In COMSOL Application Library, there is case about waveguide adapter. But there are some questions: 1) In boundary mode analysis, it just set to find only 1 mode and the S-parameter is...

12 January 2021 9,383 2 View

Generate a structure file through PGDSpider?

Hi, I am trying to generate a structure file through PGDSpider, for a diploid organism, where each locus is in two consecutive columns in one row, rather than data for each individual be stored...

29 December 2020 5,169 1 View

Why the treatment days for the xenograft model is less than the observation days?

In some experiments for investigate drug effects for in vivo xenograft model, some authors onle give treatment to the mice for 14 days or 21 days, but the data collection/observation (such as...

13 December 2020 525 2 View

Can distributed reinforcement learning realize distributed learning process?

The current distributed reinforcement learning framework, such as IMPALA, uses the structure of centralized learning and distributed execution. I feel that this is not really distributed. Is there...

02 December 2020 5,598 4 View

Is there any possible stabilize metal-doped blue-emitting light perovskite through self-assembly method?

Now, I have got a metal-doped perovskite in which blue shift happens result. I tried to make it a more blue shift to get a stable and very blue perovskite. So I think is there any possible...

04 November 2020 8,384 3 View

Why perovskite thin film PLQY much higher than dispersion PLQY?

I doped Mn into perovskite. I found when up to the best doping amount, the thin film PLQY much higher than the dispersion PLQY in my experiment. I don't know what caused it, is there any theory...

04 November 2020 2,477 3 View

Is there any relationship between crystallinity and PL indensity of perovskite?

I added the Mn dopant into the MAPbBr3 perovskite. When Mn dopant I added up to a certain amount, pl intensity can get a high result, but in the next day, the highest result I got decreased...

05 October 2020 9,192 7 View

How do I change to make an insoluble recombinant protein to a soluble protein?

How do I change insoluble recombinant proteins to soluble proteins? My recombinant protein is Aldose dehydrogenase from bacterial with around 35.0 kDa size. Now, My expression vector and...

05 October 2020 1,840 2 View

Why PLQY of perovskite can decrease too much the next day?

I tried to use Mn dopant into MAPbBr3 perovskite, but,I don't understand why the plqy of the thin film will decrease too much in the next day and then after several days it can also get increased...

04 October 2020 3,464 4 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

How to replicate the well conditions of a crystal hit with 20%(w/v) isopropanol?

I had a crystal hit in a well with 0.1M HEPEs pH 7.5, 10%(w/v) PEG 4000, and 20%(w/v) isopropanol. Why is isopropanol in w/v here if it's just solvent? How do I recreate this well condition?

25 February 2021 9,761 1 View

Problem with ligating 2.8 kb insert into 8.5 kb plasmid?

I am trying to clone 2 copies of the same mammalian gene into the pSF-CMV-Ub-Puro Ascl (contains HindIII, KpnI and NheI cut sites) plasmid. This is what the final product is supposed to look like...

24 February 2021 6,310 3 View

What urea buffer can i use for biotagged protein extraction from streptavidin beads?

Hi all, I have been doing research on biotagged LDB1 proteins in Mel cells. I purify the proteins using M280 streptavidin beads. The yield is very low so I've been trying to optimize it by...

24 February 2021 5,749 3 View

Genescan gives 3 peaks instead of one?

Hi all, I am doing a genescan analysis for a deleted exon in the patient sample. The amplified region is 215 bp. In the patient sample because of homozygous deletion, no peak is observed while in...

23 February 2021 5,645 1 View

Can I use a protein-devoid condition as my negative control in ELISA?

Hello, I am running a sandwich-type ELISA where I am trying to inhibit a protein-protein interaction using small molecules. For each experiment, I determine my Z' (Z-prime) to determine the...

23 February 2021 7,429 1 View

Frederic Lepretre

Hi Zhou Shumin

with the entire sequence of your RNA (with or without the space), you can submit it to ORFfinder software from NCBI (https://www.ncbi.nlm.nih.gov/orffinder/) and dig in the results.

all the best

fred

Tomáš Hluska

If I understand correctly, all you need to do is to clone your RNA, sequence and translate into protein in any MolBio program.