How to assess whether alternative splicing affects protein translation?

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Why the fibroblasts transfected with puromycin plasmids are dying after treatment of puromycin?

Hello, everyone. I would like ask one question. I transfected fibroblasts with EF1A-myod-puro using lipofectamine 3000. Promoter for puro is CMV. After transfection 48 hours, I did puromycin...

23 June 2024 4,902 3 View

Stable transfection be combined with two or more antibiotics to screen cells?

Can positive cell screening after stable transfection be combined with two or more antibiotics to screen cells? Will all the cells die?

10 June 2024 9,739 0 View

What role can community engagement play in heritage preservation and interpretation?

To examine the impact of community engagement in heritage preservation and interpretation

01 June 2024 3,477 11 View

Can sulfate dextran be a substrate for dextranase?

Hello every, I am looking for a commercial enzyme that can degrade sulfate dextran. What is known at present is that dextran can be a substrate for dextranase. so I want to know if sulfate...

23 May 2024 1,471 0 View

[Fairclough’s three dimension (Description+interpretaion) + content analysis ]+ [Fairclough’s (explination) +Willig’s practice +Foucaldian CDA]?

In order to understand whether the two newspapers marginalised one group and diverted attention from other group following a case study. Firstly using first and second levels of Fairclough’s...

23 May 2024 3,207 5 View

Is it easier to couple polypeptides after a few days of dissolving them in PBS?

I want to couple the polypeptide to a cell culture plate, is it easier to couple the polypeptide after a few days of dissolution in PBS?

21 May 2024 9,683 1 View

Do cultured embryonic stem cells or iPSCs migrate spontaneously?

Do embryonic stem cells or iPSCs cultured in vitro on normal culture plates migrate spontaneously without any stimulation?

18 May 2024 6,860 2 View

Why the lateral resolution of OLS5100 (Olympus) can be down to 120 nm?

The laser confocal system of OLS5100 (Olympus) can reach a lateral resolution of 120 nm, that is far beyond the diffraction limitation. Though the numerical aperture of its system is 0.95, the...

15 May 2024 1,458 3 View

How do the Maxent use to prediction the distribution of the cave fishes in Karst area?

As the known, there were the most richness species diversity of the cave animals, especially for the cave fishes, because the underground river sytems. But there were the diffculties exist also,...

14 May 2024 9,131 0 View

Preparation of PC solution?

Hello, everyone, recently I have been transferring two-dimensional materials, using PC transfer, but there is a problem with the configuration of PC solution recently, and I cannot pick up the...

11 May 2024 6,564 0 View

Is there an English Translation of the Carl Moller text: ZUR VERGLEICHENDEN ANATOMIE DER SILURIDEN?

I recently came across an anatomy text by Carl Moller that was published in 1915 but it is in German or Dutch neither of which I can understand. I would like to know if there is an English...

10 August 2024 4,346 1 View

Why does my protein refolded to beta sheet during thermal denaturation analysis?

Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...

06 August 2024 1,987 3 View

Transfection in HEK293T cells?

Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...

31 July 2024 9,891 4 View

How to retain the a GFP tagged gene expression in stable cell line?

Hello , I established a stable cell line expressing GFP tagged to a centrosomal gene having G418 drug selection marker. I validated the stable line by IFA and Western blotting, results are fine....

29 July 2024 5,005 0 View

What is the relationship between protein structure and N or C terminal tagging choosing?

I want to do 2,3-butanediol dehydrogenase(BDH) enzyme purification to confirm its activity for 2,3-butanediol. Before that, I need to confirm which N or C terminal tagging is better for enzyme...

28 July 2024 365 3 View

Why cannot i find my protein on cell surface after antibiotic selection of expressing plasmid?

I cannot confirm cell surface protein expression by flow cytometry even after transfection and antibiotic selection of cells. Does it take long for proteins to express on the cell surface? the...

28 July 2024 3,178 3 View

EDC carbodiimide and NHS easter 's half life among different pH?

It has been long known that EDC carbodiimide and NHS easter's half life agains hydrolysis are highly related to pH. However, very surprisingly, not yet found a paper giving a table/chart of their...

25 July 2024 8,737 0 View

Does post-translational protein modification cause devisions on observed pI verses calculated pI?

In running two-dimensional gel electrophoresis on bacterial protein, some spots that appear to match a protein sequence have a significantly more acidic isoelectric point than the calculated pI....

24 July 2024 8,075 3 View

What is the function of fusion tag GAMGSGIQRPTST?

I found that several expression constructs of published protein structures containing an N-teiminal fusion tag GAMGSGIQRPTST. What's the founction of this fusion tag?

23 July 2024 1,352 4 View

How to estimate a protien's size using Chimera or PyMol? how correct this estimated can be?

I need to know the approximate size of my target protein for Negative-stain EM. I have an alpha-fold predicted structure of the protein. I do not see any turtorial on internet for estimating the...

21 July 2024 5,732 3 View

Frederic Lepretre

Hi Zhou Shumin

with the entire sequence of your RNA (with or without the space), you can submit it to ORFfinder software from NCBI (https://www.ncbi.nlm.nih.gov/orffinder/) and dig in the results.

all the best

fred

Tomáš Hluska

If I understand correctly, all you need to do is to clone your RNA, sequence and translate into protein in any MolBio program.