I have done it just the way you said. It worked for E.coli when I resuspended again. I heated at 70ºC. :)

@Xi Jiang thanks for the answer. didn't you have any problem? Did your plasmid look fine on the gel?

I did it for a Ti plasmid purified from E.coli. I transformed the resuspended plasmid both in E.coli and A.tumefaciens afterwards and colonies grew and also the tobacco plants infiltrated with plasmid transformed agro expressed the correct proteins. I didn't want to heat at too high temperature because I was worried about the plasmid integrity and I did it in a thermometer not in a thermocycler as thermocycler controls the added heat according to a volume. During the water evaporation the volume of your plasmid will change and therefore, the temperature will be increasing over time.

What do you want to do with your dried plasmid?

@Xi Jiang I'm going to use it for sequencencig the insert inside the plasmid afterwards.

So long as the temperature is below the melting temperature of the DNA and you don't have lots of contaminants in the solution that could damage the DNA then it should not be an issue. After all, many thermophiles live at elevated temperature and their DNA is fine.

Paul Weber

Have you run a gel? What did it look like?