Inconsistencies in PCR results after bisulfite conversion?

03 April 2016 4,761 1 View

Should there be a correlation between band size on gel and SYBR green signal in the real time PCR run?

31 December 2015 8,981 4 View

Is there logic behind using Annexin V FACS with another antibody?

11 December 2015 7,787 3 View

What are the alternatives to 5-azacytidine for bursal B cell cultures?

10 November 2015 4,685 3 View

High miniprep yield followed by extremely low Ct values?

I have been using Clone jet PCR cloning kit with a pJET1.2 blunt as a vector. The insert is from a clean PCR product that went through a blunting reaction (supplied with the cloning kit). The E....

09 October 2015 981 7 View

How do I use two way ANOVA on real time PCR results?

02 March 2015 5,245 7 View

How do I calculate standard errors for normalized log2 converted gene expression data?

I ran an experiment with a ref gene (3 biological repeats x 3 technical repeats) and normalized gene expression of my target genes to the ref gene. I decided to present the data as log2 fold...

31 December 2014 8,722 7 View

Has anyone had any experience with storing cell culture slides for a TUNEL assay?

11 December 2014 3,525 2 View

Does anyone have a specific recommendation for a DNA pol or mix fit for PCR ?

I'm using bisulfite treated DNA as template for sequencing purposes.

08 September 2014 6,737 7 View

Any problems using the same primer set twice?

Theoretically, is there a problem with running a PCR and then running the same system again, with the same primers, with the product of the first reaction to further amplify the product? The...

07 August 2014 5,355 8 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

Chlorinated organic formation in odor control scrubbers using hypochlorite?

I'm involved in a study of odor control technologies for municipal wastewater treatment plant. One of the control options involves a chemical 2-stage (acid/alkaline) packed bed scrubber. The...

03 March 2021 3,661 2 View

What solvent can I used for Paal Knorr reaction between 6-amino hexanoic acid and 2.5 hexanedione?

I am try to make the Paal Knorr reaction between 2.5 hexnedione and 6-amino hexanoic acid, my problem is type of solvent to use, because 6-hexaminohexanoic acid is soluble in water but I am not...

02 March 2021 4,443 2 View