Could you suggest a chitosan/TPP nanoparticles degelation method?

03 April 2017 1,922 3 View

How do you account pre-detached cells in trypan blue assay?

07 August 2016 8,860 5 View

What happens to cells treated with TNF/CHX/zVAD/Necrostatin-1?

07 August 2015 3,275 2 View

Have you ever used trziol/Tri reagent/Qiazol with adherent cells grown in plastic flasks?

Dear colleagues, so the question is in the title... Background: we've spent a few days trying to isolate RNA from an adherent cell culture grown in a 6-well plate. Isolating RNA from various...

02 March 2015 9,250 12 View

What does the multifactor dimensionality reduction whole dataset statistics show?

02 March 2015 319 4 View

How do xenografts metastasize?

Dear colleagues, I've browsed a lot of papers and encountered the fact that when the both primary and metastatic tumors are of interest for researchers and when the xenograft model is involved,...

01 February 2015 3,280 7 View

Custom organic synthesis services

09 October 2014 4,639 0 View

Can anyone suggest whether transfection medium must be replaced 24 h after transfection?

Dear colleagues, should I change the medium after transfecting HepG2 cells with the RNAiMAX reagent for 24 hours? The supplier's protocol does not include this step in the knockdown assay, while...

06 July 2014 4,379 12 View

Can you share your knowledge on oxidized nucleobases catabolism?

Please share your knowledge on the faith of oxidized nucleic acid bases. Fast yet extensive PubMed browsing gave no positive results. Most papers deal with processes that lead to free oxidized...

02 March 2014 9,817 1 View

How many scrambled siRNAs do you use?

Dear colleagues, how many scrambled siRNAs do you use in case when you either target single gene with two or more siRNAs or target multiple genes with multiple siRNAs?

01 February 2014 4,028 7 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Chlorinated organic formation in odor control scrubbers using hypochlorite?

I'm involved in a study of odor control technologies for municipal wastewater treatment plant. One of the control options involves a chemical 2-stage (acid/alkaline) packed bed scrubber. The...

03 March 2021 3,661 2 View

What solvent can I used for Paal Knorr reaction between 6-amino hexanoic acid and 2.5 hexanedione?

I am try to make the Paal Knorr reaction between 2.5 hexnedione and 6-amino hexanoic acid, my problem is type of solvent to use, because 6-hexaminohexanoic acid is soluble in water but I am not...

02 March 2021 4,443 2 View

Is there a powerful system for the security of the systems distributed on IoT systems?

Is there a powerful system for the security of the systems distributed on IoT systems?

02 March 2021 3,858 10 View

How Relativistic effects is one reason for lanthanide contraction?

The Lanthanide contraction which is the decrease in ionic radii of the elements in the lanthanide series from atomic number 57, lanthanum, to 71, lutetium, is due to poor shielding of the 4f...

01 March 2021 2,272 4 View

Ester cleavage conditions?

Hi all, I need to cleave the ester in Ethyl 2-amino-1H-imidazole-5-carboxylate to make the carboxylic acid and retrieve the product from solution to use in downstream applications of linking to...

01 March 2021 8,766 3 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

How to remove DCU and HOBT from my reaction?

I am doing a acid and a chiral amino alcohol reaction using DCC and HOBT in DCM or THF? Can anyone suggest me how to remove DCU and HOBT from a reaction mixture??

28 February 2021 2,153 1 View