Conducting a survey to understand the response to work from home during the covid-19 lockdown?

Greetings, We are conducting a survey on work from home and its impact, please fill the questionnaire and contribute to the growth of academic understanding of the concepts and for policy...

03 May 2020 3,758 22 View

What are recommended temperature conditions for purifying his tagged proteins under denaturing conditions(GuHcl)?

Hello everyone, I'm trying to purify his-tagged protein under denaturing conditions using Guanidine Hydrochloride (Protocol from GE Healthcare-attached below). Though I'm getting my protein in...

10 December 2019 8,654 3 View

How to access stress/ strain at integration points in a umat code?

Im trying to code a damage model for beam elements, for which I know the strain at rupture. I was wondering if I could access the strain at the center of the beam to start and progress damage. As...

28 May 2019 9,755 1 View

Can any one help me to get the MATLAB code for cluster head selection and routing in wireless sensor networks using PSO and GA?

wireless sensor networks- optimization techniques

10 May 2019 2,944 1 View

Hiii every one i tried many time but my pcr negative control is contamination?

I changed new primers and water and anealing temperature but i didn't get results?

11 September 2018 3,512 3 View

What do you think about such journals, which just publish whatever an author submits?

I was reading through this article. Just felt very bad about the contents which are published by some journals. Looks like there is a lot of garbage, which needs to be identified and...

22 December 2017 3,471 25 View

How much is 1 volume of 70% ethanol?

I need to isolate RNA from the tissue sample. The procedure is given like "add 1 volume of 70% ethanol to the lysate, and mix well by pipetting". How much ethanol should I add?

19 November 2017 8,272 5 View

Bacterial intracellular synthesis of nanoparticles,Could anyone suggest any standard bacteria(preferably gram negative bacteria)for NP synthesis?

Hi, this is part of designing a practicles for students, could anyone suggest any bacteria mediated intracellular synthesise of any nanoparticle (10-50nm) and that gives highly repeatable...

13 December 2016 3,835 4 View

What is the method for separate out the mixture of Ethyl acetate and tetrahydrofuran ?

30 June 2016 9,968 9 View

How can I conduct SEM sample preparation (Compacted Bentonite soil)?

My aim is to study the micro-structural changes in compacted bentonite for different moisture contents w.r.t time.For that I need to conduct SEM. So, please suggest the precautions I need to...

27 January 2016 5,703 2 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

Can linear DNA be used as template for site-directed mutagenesis?

Is it possible to induce site-directed substitution mutation by quick-change method on linear dsDNA? or it has to be cloned in some vector? If yes, should it be treated with the Dpn1 enzyme...

03 March 2021 401 4 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Chlorinated organic formation in odor control scrubbers using hypochlorite?

I'm involved in a study of odor control technologies for municipal wastewater treatment plant. One of the control options involves a chemical 2-stage (acid/alkaline) packed bed scrubber. The...

03 March 2021 3,661 2 View

What solvent can I used for Paal Knorr reaction between 6-amino hexanoic acid and 2.5 hexanedione?

I am try to make the Paal Knorr reaction between 2.5 hexnedione and 6-amino hexanoic acid, my problem is type of solvent to use, because 6-hexaminohexanoic acid is soluble in water but I am not...

02 March 2021 4,443 2 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View