What control do you need for polysome profiling experiment ?

Philippe Paget-Bailly @Philippe_Paget-Bailly

10 October 2019 1 5K Report

Hi everyone,

I was wondering what antibodies or pcr primers I would have to use to control my fractions in a polysome profiling experiment ?

A quick search highligthed antibodies against small and large ribosomal subunit like rpl17a and rps3. And anti-Ssb1 for soluble fraction ?

Can you me give me advice on this topic please ?

Thanks,

Philippe.

Poonam Pandey

Hi Philippe,

For western, you can use rpl17a and rps3. For PCR, we generally use any housekeeping genes such as gapdh, actin, hsp 90 for normalization.

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