Hi all,
Am working on a DNA based biosensor, DNA are thiolated. i reduce thiolated ssDNA by TCEP and dip cast it on a gold nanoparticle coated electrode.
I have tried with 500nM , 1uM, 2uM, 3uM, 4uM and 5uM concentrations, but am not getting a constant value by DPV.
i use methylene blue as indicator, should i have to refrigerate the methylene blue after use.
i need to fix the probe concentration.
Can some one share their insights on this, that would be great help.