appeared after one week of culture , it is round and white at the at the bottom of a dish and once it appeared after one day
Generally, this kind of contaminants are found when the culture plate is too old (≥6 months) or handled inappropiately. Normally yeast are found in our body and a part of healthy mix of normal flora. If I am not mistaken, it looks like yeast contamination; avoid unwanted movements, speaking while plating. Keep your work bench clean and wipe with 70% ethanol.
Hi
Without knowing your culture conditio s and having some of my experiments in mind could it be that this is crystallization of salts or carbs in your medium? This looms a bit like my fixed cells when I stored them under PBS and the plates got dried accidentally
Sven
Hello
You have fungal contamination. Fungus and Yeast are the common contaminants in culture as at times due to improper sterilization and long storage of culture media or tools.
Good Luck
Follow proper Risk Assessment guidelines: Good Laboratory practices...
I don't see any cells. Is this "contamination" on the same plane of focus as the cells? If not, then have you tried wiping the underside of the culture dish? It could be salt crystals from evaporated media contacting the underside of the dish.
Then it is not IN the plate but instead is under the plate. That means you do not have a contamination in your culture.
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