PCR, Cloning, Restriction Digestion, Ligation, Transformation, Plasmid et al | Contact experts in Molecular Biology to get answers
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Questions related to Molecular Biology
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Hello everyone, we are acquiring a new molecular biology laboratory with the fundamental objective of identifying species of microalgae and cyanobacteria. Perhaps you could help us decide if it is...
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Probably a silly question but I am a complete novice in molecular biology. I have decided to attempt a phylogenetic analysis. I have managed to create a phylogenetic tree but want to confirm...
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15 January 2021 4,717 8 View
All of the papers I have seen regarding the expansion media for HUDEP-2 cells have used StemSpan (StemCell Technologies). However, there are other cheaper "Hematopoietic Stem Cell Serum Free...
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I made a new bacterial strain with an unmarked deletion using 2 step homologous recombination (with sacB counterselection). The full protocol is given in the link at the bottom. The protocol...
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Since two decades we came across many tools to edit the desired gene and genome viz., clustered regularly interspaced short palindromic repeats (CRISPR)-CRISPR-associated protein 9 (Cas9), (2)...
28 December 2020 7,929 10 View
Right now, I want to design qPCR primer for my target gene. But that gene has 21 predicted mRNA sequence in the NCBI database. Most of the sequence names are same but only different location...
24 December 2020 5,841 12 View
Dear All, The forward and reverse sequences obtained from the PCR products are not identical at all. Can I still make the contigs for doing BLASTn? Individually both sequences are showing the...
23 December 2020 5,666 5 View
It is known that the Uracil-DNA Glycosylase can remove the basic residue of dU. Thus the DNA chain will further cleave at the point of dU when heating to 95℃ However, is there any method to cleave...
19 December 2020 7,243 3 View
Dear all, My question is about Inducible hypoxia in vivo (mouse model). I'm looking for some chemicals that can induce the pathway of hypoxia or some other techniques. If you now know something...
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have a safe and healthy days i want to know what is the best solution to be used instead of HBSS solution in comet assay while using g bone marrow samples thanks
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I studied biochemistry and molecular biology in my bachelors and Immunology in my masters. I never came across a suitable course for general signaling pathways and principles. My interest is...
14 December 2020 5,971 5 View
I was using molecular grade water for the qPCR experiment. I want to know if I use autoclaved double distilled water is it fine? What about using DEPC water? Please share your knowledge and...
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Hello, everyone. what are the limits of molecular biology? especially when we used it in the identification of the species. Thank's for your answer.
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We are fabricating acrylamide beads as probe substrate. How can we make streptavidin bind to the surface of the acrylamide? Thanks!
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After doing my primer efficiency, my lowest dilution cDNA ct value is 20.16 and NTC ct value is 31.76. The melting curve is the same for target product and NTC and it is giving the same product...
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Hi! We are designing a genome probe, which need to be cut off the acrydite substrate. Yet the photocleavable spacer is too expensive (＄500 for 50nmol primer, which is just 1 time of assay). Is...
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