PCR, Cloning, Restriction Digestion, Ligation, Transformation, Plasmid et al | Contact experts in Molecular Biology to get answers
1,961 views 3,277 posts
Questions related to Molecular Biology
We are
13 May 2024 1,640 1 View
I have loaded mesoporous silica nanoparticles with a drug and attached them to antibodies but after conjugation with antibodies, the nanoparticles loaded with the drug lose their functionality to...
13 May 2024 4,658 1 View
I am checking GFP fluorescence level in heme sensor. 200 ul of sample was loaded in black flat bottom 96 well plate in H1 Synergy machine. Temperature was 30 degree. I am using S. cerevisiae WT...
07 May 2024 5,716 3 View
my gene of interest size is about 4kb. I used hi fidelity taq pol as well as Q5 from NEB but still its happening. my forward primer composed of a 4 adenosine then restriction site followed by 6x...
06 May 2024 4,527 2 View
Is there a convienient way to mark cancer cells that are undergoing mitosis?
06 May 2024 8,137 4 View
The common Tm without Restriction site is 61 degrees and the Tm with Restriction site is 71 degrees. The reaction conditions i followed is 95 degrees 3 mins, 95 degrees 30 secs, Tm (60-68) degrees...
03 May 2024 500 3 View
For an assay I am planning to run I require a glucose-free MEM-alpha medium, I've seen papers where researchers state they use glucose-free MEM-alpha but they do not provide a supplier or catalog...
03 May 2024 3,976 0 View
I`m in project about miRNA in function, mutations, process and other for explain in a class. And I`m a autism, I have difficult to understand if the peoples the class understanding my class. So, I...
02 May 2024 2,570 0 View
I am trying to optimize sonication for ChIP of AML cell lines viz. HL60 U937 THP1. I am fixing cells with formaldehyde and sonicating 2.5 million cells at a time. If anyone has worked with these...
01 May 2024 7,684 0 View
I observed this clumps in my MCF-7 cell culture. Does anyone know if it is normal or contamination?
30 April 2024 9,617 3 View
In terms of communication, Gnasthenomus petersii has been compared to songbirds, bats, elephants, dolphins, whales, as well as humans. On this point, Chomsky (2012) believes that the ability to...
28 April 2024 1,055 0 View
Should I use multiple gRNA transfections (Donor) mixed as well as one by one along with a Cas9 plasmid to see which gRNA is working, or start with only 1 gRNA at a time? Options: 1 gRNA + Cas9 (1...
25 April 2024 9,213 3 View
I regularly have low DNA concentrations on the vetigastropod tissues I extract. I have tried fresh tissue and older/museum tissues. We use the Thermo Scientific GeneJET Genomic DNA Purification...
09 April 2024 3,580 4 View
I am running ELISA for different biomarkers against meningitis. I need to know how to calculate cutoff values for it in ELISA. There is no negative control or positive control for these kits,...
08 April 2024 3,027 1 View
Hi, I have recently been working on RNA purification from RNA-binding proteins. I used a protein-specific antibody to immunoprecipitate the protein RNA complex and then extracted RNA from the...
02 April 2024 5,103 1 View
I have a plasmid with kanamycine antibiotic resistant gene and Bar as a marker gene. I need to transfer this plasmid into AGL-1 strain of agrobacterium tumefaciens. I faced a problem when I follow...
27 March 2024 3,860 4 View
Recently, people working in faunal research have practice to photomontage when identifying a species (in most cases they are not professional taxon experts), and they make a mirror image of one...
22 March 2024 6,642 8 View
The samples are 250 μl serum mixed with 750 μl Trizol Isolation by (Chloroform Method ) Homogenize the sample Add 200 μl Chloroform and mix well then incubate on ice for 15 min, then centrifuge...
20 March 2024 6,755 3 View
Hello everyone! I want to know about research square.Is it okay to submit manuscript to reseach sqaure before submitting to a journal. Actually I have no idea about research square
18 March 2024 2,657 3 View
Hello. I'm making non-denaturing PAGE of DNA fragments for analysis of polymorphisms, but I'm getting wavy bands. If somebody can help me, I will be very greatful. First of all, here's my recipe...
16 March 2024 7,664 1 View
What is genetic restoration of endangered species and why species and genetic diversity are each important for an ecosystem?
13 March 2024 5,806 2 View
I have purchased 'Casein fluorescein isothiocyanate from bovine milk, Type III'. However, I cannot find any literature that defines the Type III caseins. The product documents also do not provide...
11 March 2024 6,785 3 View
Hello everyone, Can anyone please let me know how can I extract DNA of Cyanobacteria (Gram Positive bacteria) in on site?
10 March 2024 2,781 1 View
What is the best way to find a bunch of email addresses related to academics working on "Plant Endophytes"?
08 March 2024 5,366 1 View