PCR, Cloning, Restriction Digestion, Ligation, Transformation, Plasmid et al | Contact experts in Molecular Biology to get answers
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Questions related to Molecular Biology
We now know that a mutation in MAOA gene excessively impact the person's susceptibility to aggressive behavior. However, research reports that findings regarding this gene were more consistent in...
20 October 2018 5,116 3 View
Dear Colleagues, I'm trying to synthesize a linear deletion cassettes using over-lap PCR, as described in the paper: B. Lesic, L.G. Rahme (2008). BMC Molecular Biology 9:20 There are three steps...
17 October 2018 3,907 6 View
Hello all! Does anyone know a protocol that i can use to determine whether a medium contains any fatty acids or sterols? I don't really need to identify or measure the levels of these...
19 September 2018 718 3 View
Hi fellow scientists, I am researching a gene in Enterococcus faecalis, classified in two groups: 1) Penicillin-sensitive/ampicillin-sensitive (Control) and; 2)...
18 September 2018 9,886 4 View
research project
12 September 2018 3,473 9 View
I have observed that BbsI (and this phenomenon occurred with both the original conventional form, as well as the High Fidelity; note, enzymes were ordered brand new, handled properly i.e....
06 September 2018 4,353 3 View
What are the molecular biology techniques used to modify a given ligand-peptide sequence?
01 September 2018 3,156 3 View
I am curious about how the ratcheting of a DNA strand through a nanopore works in (oxford) nanopore sequencing. I think that the enzyme motor is first attached to one end of the DNA strand to be...
31 August 2018 7,871 1 View
Hello, I would like to know why some vectors contain this AAV2 ITR sequence, I want to remove that sequence together with others of my vector to make the stable transfection with lipofectamine...
16 August 2018 7,568 4 View
The pcr mastermix brand is 2x mytaq HS red mix - bioline
10 August 2018 5,719 23 View
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Dear all, according to your experiences what is a better approach for non-model plant RNA-seq - refererence based assembly or de novo assembly? I will do RNA seq experiment in Papaver somniferum...
31 July 2018 7,987 5 View
Hi Dear colleagues, i had registered 3 DNA fragments in NCBI through DDBJ gene bank , i understand that the two gene banks in addition to EMBL gene bank are linked and the data is interchanged...
22 July 2018 7,387 1 View
Many researchers tend to register the DNA sequence that they work on in NCBI or DDBJ or EMBL. What is the benefit of the registration for the researcher and for in general and what is the...
20 July 2018 5,191 3 View
in the synthesis of TaqI endonuclease using Ecoli strain by NEB company for example, where from they get the gene coded for this enzyme, what is the function of this enzyme in the original...
08 July 2018 8,005 3 View
Hello all, I am wondering if there is any cheap method for plant cell wall disruption and getting DNA, RNA and proteins into solution. Obviously, there are many standardized ways: Liquid...
24 June 2018 4,374 10 View
Is there a way to automate such a process whilst having all the additional data of the sequence stored in a nice legible manner?
13 June 2018 1,470 3 View
Hello everyone! I am currently having problems with my Fw and Rv primers (including restriction enzymes) to subclone my target gene to pcDNA3.1. I have designed the Fw from the Start codon and Rv...
13 June 2018 1,692 9 View
Recently I have read interesting paper " The triplet genetic code had a doublet predecessor". Patel A. The triplet genetic code had a doublet predecessor.... What do you think about this...
10 June 2018 6,956 5 View
I am trying to perform a rather difficult blunt-end ligation that requires several enzymatic steps and would appreciate any tips from those who have experience with blunt-end cloning. My...
07 June 2018 3,959 10 View
This was sparked by a quote I read ' The way that scientific publishing fetishizes novelty is gross. That's the business model of tabloids, not scientific journals. ' -Ed young
01 June 2018 516 5 View
Hello, I am wondering if could be possible to align set of protein sequences (for example 100 protein sequences) each to each by any user friendly way. I.e. sequence no. 1 with the sequence no....
30 May 2018 8,155 10 View
My research objective is to silent a targeted gene through RNA interference (RNAi). I cloned my designed gene fragment (double stranded RNA) in L4440 vector under the control of two...
25 May 2018 10,089 5 View
From what I've seen most people use either manual extraction, Norgen Biotek's phage DNA isolation kit, or Promega's Wizard DNA clean up system. I was wondering if the Qiagen Dneasy blood and...
24 May 2018 7,936 6 View