PCR, Cloning, Restriction Digestion, Ligation, Transformation, Plasmid et al | Contact experts in Molecular Biology to get answers
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Questions related to Molecular Biology
I recently got plasmid from Addgene, however, I failed to expand them into glycerol stocks within 2 weeks, as recommended. It had been two months since I got the plasmid, and in the middle our 4...
09 November 2022 6,237 5 View
I am running an MD simulation in Desmond. It runs perfectly when started from the graphical user interface of the program. I tried to perform it from the command prompt. I saved the cms and cfg...
01 October 2022 7,334 0 View
Hi, I want to extract proteins from the skeletal fibers of adult mouse. To decide how many legs contain enough proteins for downstream experiments , I want to know the number of skeletal fibers...
01 May 2022 1,182 0 View
Hello together, I am currently looking on literature about the impacts of natural gas, coming from leaks, spills etc., on marine biology (fish, marine mammals, birds, plants etc.). Is this topic...
28 February 2022 3,755 3 View
How do I find out which region of a given nucleotide sequence (encoding an entire protein) encodes for a particular region of the protein?
15 November 2021 439 4 View
Hello, I have been seeing a strange contamination in my cells I have not been able to solve. It is Dictyostelium cell culture and therefore anti-fungal cannot be used. Cells have been cultured in...
02 November 2021 3,683 2 View
I have tried to do a 50ns of the GABA receptor (a tetrameric protein) with a ligand and after the simulation i noticed the different units of the GABA receptor moving about as i was playing the...
29 October 2021 3,771 1 View
I am looking for papers with detailed methods on what molecular biology techniques are available for engineering mushroom-forming Basidiomycetes. I know that they are more difficult to manipulate...
27 September 2021 7,956 4 View
What exactly we need to build a molecular biology lab from scratches? What are the basic minimum requisites? What was required 15 years back is now obsolete. What will we require to study the...
09 September 2021 4,751 5 View
Losartan drug having aryl tetrazole and primary alcohol units. I am interested in intermolecular amination reactions. How to do selective protection of primary alcohol over aryl tetrazole nitrogen?
28 August 2021 1,927 2 View
I am currently working with U87MG (glioblastoma) cells. I am using MEM with NEAA+ 1mM Na Pyr+ FBS as media. The cells grow fine in T-25 flasks. But sometimes, when I check them, they seem to lose...
27 July 2021 5,731 3 View
We could not get the data that Genclone uses to draw the Pareto chart. We want to draw a high-quality figure of the graph because the software produces low-resolution figures that do not meet the...
29 June 2021 2,965 2 View
I am planning to have endonuclease Ⅳ and Bst/Taq work in the same buffer, but definitely in different Temperature, i.e, the system will be passing first 37℃ then 60/95℃. Can I simply put...
14 May 2021 3,311 3 View
Hi All, I tried to clone 12 gene, about 1kb, including ploy A (40) into pUC57 individually, using NEB stable cells, but only 2/12 have successful clones, the other 10 can't get the correct clones,...
07 May 2021 6,983 0 View
The EasySelect Pichia expression manual by Invitrogen says zeocin is not needed after clone selection, but when we express with zeocin we get lots of protein production and when we express without...
04 May 2021 6,852 6 View
I need to test if a certain value (e. g. the number of lung cancer patients) has changed over time (e. g. over the last 30 years). I want to observe time trends over the years – when did the...
03 May 2021 3,767 3 View
I am creating CRISPRi tool for Lactococcus lactis. Do I need to add an extra Lactococcus Terminator sequence at the end of the sgRNA or is the S. pyogenes Terminator enough?
28 April 2021 9,479 3 View
I have taken slices of a fresh frozen brain, stored at -80C, using a CryoStat set to -8 C. I then took specific regions of the brain using a micropunch and immediately ejected them into RLT buffer...
28 April 2021 1,523 3 View
I've began working with the immortalised human myoblast cell line LHCN-M2. I've done a differentiation protocol and morphologically under the microscope its worked really well. I tried to run an...
26 April 2021 7,603 7 View
Fully funded Post-doc looking for host lab. I've a PhD in biotechnology and molecular biology. I need approval letter to submit. For cv DM on [email protected]
24 April 2021 9,902 4 View
Hey everyone, I am looking at the influence of systemic inflammation on myelopoiesis. Does anyone of you know a suitable model system to differentiate human iPSCs (induced pluripotent stem cells)...
13 April 2021 6,634 1 View
We are using the real-time cyclers Applied Biosystems model 7500 for our qPCR measurment. The issue is that we have gotten a order with the wrong format of 96 well plates, we got plates designed...
24 March 2021 9,310 0 View
Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...
02 March 2021 8,821 2 View
We made the global transcriptome (global mRNA expression), by RNAseq, from human subcutaneous adipocytes. Subsequently we did the Western blot for some proteins that were determined from the...
22 February 2021 5,025 6 View