PCR, Cloning, Restriction Digestion, Ligation, Transformation, Plasmid et al | Contact experts in Molecular Biology to get answers
1,482 views 2,910 posts
Questions related to Molecular Biology
I came across a term like "non canonical translation in RNA viruses". What does the term canonical mean?...even though after a dictionary search I havent got a satisfied answer. Does the word...
28 January 2018 6,277 4 View
The pet28 is a very low copy plasmid. I cant get a pellet in isopropanol step. I have to use a mini spin kit Qiagen a to do fraction of 10 ml.
26 January 2018 5,259 3 View
Do you know anything about possible interactions between proline and mGluR5 glutamate receptor ? I have found that proline interferes with other glutamate receptors, but nothing about mGluR5, but...
25 January 2018 5,804 4 View
More than 2 years ago, Tomasetti and Vogelstein brought that question to prominence when they tried to sort out environmental versus inherited causes of cancer. They examined the extent to which...
22 January 2018 2,174 9 View
I digested insert (in pJET) and pNIP40b (mycobacteriophage MS6-derived) vector with XbaI; then I transformed the ligation (ratio 1:1 and 1:3) and the linearized vector (control) in Stbl2. After...
18 January 2018 9,040 3 View
I need to find the location of the gene associated with particular character from the genome, also I have no clue weather the gene is present in plasmid or in chromosome of bacteria. Can someone...
18 January 2018 6,011 8 View
NAD is involved in most cellular redox reactions as an oxidant (NAD+) or reductant (NADH) and it changes between both states multiple times in every fraction of second in every cell. But it is...
12 January 2018 2,362 13 View
Dear all, I am currently trying to do a 3-way Gibson Assembly using 3 DNA fragments of size, 2.4 kb, 4 kb and 6.3 kb. The final ligated product will have a size of 12 kb. My protocol involves...
09 January 2018 4,276 5 View
Dear Researchers, I have purchased my designed oligos from Sigma for miR based silencing studies as sense (~64nts) and antisense (~64nts); together if annealed it is ~64bp. I take equimolar...
09 January 2018 8,027 4 View
After the chemical or UV mutagenesis, the yeast culture is supposed to be plated on the selective medium with canavanine or 5-FOA in order to assess the efficiency of mutagenesis. The questions...
05 January 2018 4,900 3 View
Functional genomics is a field of molecular biology. What are the common and uncommon tools between them?
02 January 2018 9,446 3 View
Can molecular biology vectors like puc 18 or pGEMT be stored at minus 80 degree?
30 December 2017 6,457 3 View
Hello. I am studying on plant breeding. In my lab, the others usually are performing researches related to molecular biology and genetics. As far as I know, general process of research is studying...
21 December 2017 8,962 2 View
I would like to prepared a DNA sample that has -SH or thiol group at one of its end so that I can covalently immobilize it on a surface. The -SH modified primers are commercially available. I am...
20 December 2017 5,684 6 View
Dear all, I am involved in a small project to investigate the way scientists communicate their work and are engaged in social media. We have created a small survey, which you can find following...
02 December 2017 3,706 11 View
I am working on a time course expression analysis experiment with developing Arabidopsis siliques. I am facing difficulties in extracting RNA from the limited amount of sample (approx. 30-50 mg of...
02 December 2017 3,120 3 View
I would like to know where do you usually send to sequencing since I am looking for cheaper prices than those ones that we have here in Mexico (I would like to do sanger sequencing). I hear that...
21 November 2017 1,241 3 View
Dear All, I have synthesised cDNA first strand using SuperScript III (Invitrogen) and would like to amplify my gene of interest for overexpression studies? Is it necessary to synthesis second...
09 November 2017 6,003 11 View
Hello everyone, I've started doing molecular biology, rather recently. So, I've spent some time to obtain my first sequences at ABI platform and all of it were perfectly readable wheteher seq....
07 November 2017 8,494 6 View
I have purchased human histone octamers from Sigma (SRP0408) for nucleosome reconstitution experiments. I would like to check the protein complex in octameric form. Running it on SDS-PAGE will...
31 October 2017 3,386 5 View
I am trying to stitch in a 38 amino acid tag to the N-terminal end of my protein (3200bp) to be cloned into a lentiviral vector (~7000bp). The forward primer for the same, along with the overhang...
26 October 2017 4,393 6 View
Hello everyone, I isolated DNA from freshly taken Human blood but during processing I was getting RBC contamination in pellet .I washed with RBC lysis buffer 6-7 times followed by...
17 October 2017 7,699 3 View
Plant Morphology versus Molecular phylogeny
09 October 2017 2,433 9 View
I am trying to isolate the intestinal epithelial cells and lamina propria cells from mouse small intestine. And the thing is... I've tried several protocols from JOVE(Geem, D., Medina-Contreras,...
03 October 2017 7,413 2 View