PCR, Cloning, Restriction Digestion, Ligation, Transformation, Plasmid et al | Contact experts in Molecular Biology to get answers
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Questions related to Molecular Biology
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I'm trying to polarize human monocytes to M1 or M2 phenotypes. I cultured them for 5 days in RPMI medium +10% FCS with 50 ng/ml GM-CSF (for M1) and RPMI medium +10% FCS with 50 ng/ml M-CSF (for...
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Molecular biology
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Hi all, Simple question, maybe complex answer - is it OK to perform sub-cellular mitochondrial fractionation on cultured cells (293T etc) that have been harvested then *frozen* as pellets? Just a...
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Any synthetic analogue of Hemoglobin or any other Xenobiological agents can be cited as example. Please provide links and references.
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I have seen the antibiofilm effect of a phytochemical. It disrupts EPS but I want to know the mechanism of that agent at the genetic and molecular level . What experiments should I employ?
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I docked the GOLD program version 5.7.2 and when analyzing the interactions between the ligand and the receptor I noticed that the amino acid sequence is incorrect when saving the complex in pdb....
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So I am a new PhD student but I have been doing good research since I have been an undergrad. I have scientific interest in a lot of things and firmly believe that "A change in perspective is...
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Dear colleague I am working on U87-MG line. I have seen some new shapes of cells in my two recent subculture that were strange for me. it is passages number 7, i used DMEM high glucose containing...
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I need help on how test its antioxidant role in a molecular biology lab, procedures used.
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I was able to insert 1.1 kb goi to the bacterial genome. Now, I want to insert 3 kb fragment. I wonder if anyone had done it.
17 October 2019 8,102 3 View
Hi, I wonder if people know 3'deoxy ribonucleotide (i.e. with 2'OH) is commercially available? I didn't find any in the modified bases product section in IDT, thermo, or sigma. I'd like to use...
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Is it possible to tag GFP (Cys) modified which can be tagged with Alexa568, though it must still has GFP working? Has anybody tried? Is it conceptually and theoretically possible or there is some...
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i am a freshfish in molecular biology, recently, i am planning to do a gene mutant using the dominant negative effect. But i have no idea about this methods. so who can help me, inducing the...
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I have to perform RNA extraction with TRIzol LS and I just realized that we have chloroform stabilized with ethanol. Is that ok? And should chloroform and isopropanol be "molecular biology...
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HiMedia seems to be cheaper than the rest.Anybody who has used it for molecular biology experiments, how is the quality?
27 September 2019 7,171 3 View
I am working on S. aureus ATCC 6538 strain. I prepared electro-competent cells and tried to electroporate the plasmid into this strain. After many attempts, this strain failed to accept the...
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Dear research professors and scholars, I have developed a novel 3D protein structure (mutant DNA Gyrase enzyme of antibiotic-resistant E. coli) by homology modeling technique. Because this type of...
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I know this is a very broad question, but in your experience how sensitive is qPCR? It is reported that qPCR can detect even 1000 CFU/ml or less of bacterial species in human samples, and...
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I have the siRNA in my hands but the Accell siRNA Delivery Media will take some weeks to arrive. I am looking for an alternative medium to try to begin.
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