PCR, Cloning, Restriction Digestion, Ligation, Transformation, Plasmid et al | Contact experts in Molecular Biology to get answers
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Hi everyone I am running Structure version 2.3.4 programme smoothly.But now I am facing problem that in Result folder I am getting only one file although I am setting k=2 to k=10 and iterations...
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I have read this in a book that DNA polymerase can discriminate between ribo and deoxyribonucleoside triphosphate bcoz of steric exclusion of rNTPs from the DNA polymerase active site, as the...
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I need to format a manuscript I am writing for Journal of Molecular Biology. I am not able to find a LaTeX template for formatting the manuscript. Please help.
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I am making 200ml of 0.5M EDTA, pH8. I mixed 29.22g EDTA (MW: 292.24g/mol) with 100ml distilled water first (planning to add up to 200ml after measuring pH). I was adding NaOH pellet into the...
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I want to understand the underlying mechanism behind the above-mentioned question.
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Does anyone have any recommendations or references on how to study the pharmacodynamics and pharmacokinetics of bacteriophages using in vitro cell line assays? Should I focus on comparing or...
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I am going to construct a library in E. coli, and the number of variants is about 107. To make sure that every variant in the library is included, each plasmid should be transformed into E. coli...
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Hello, If I want to clone a gene of interest in a plasmid containing ORF, how should I do that, and which criteria I should follow? Thanks, Ramanjaneyulu
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For RNA profiling of frozen tissues, researchers recommend to use single-nuclei RNA sequencing instead of single-cell. What is the reason for this? Also, what is the best way to freeze cells for...
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