PCR, Cloning, Restriction Digestion, Ligation, Transformation, Plasmid et al | Contact experts in Molecular Biology to get answers
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Questions related to Molecular Biology
I observed this clumps in my MCF-7 cell culture. Does anyone know if it is normal or contamination?
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In terms of communication, Gnasthenomus petersii has been compared to songbirds, bats, elephants, dolphins, whales, as well as humans. On this point, Chomsky (2012) believes that the ability to...
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Should I use multiple gRNA transfections (Donor) mixed as well as one by one along with a Cas9 plasmid to see which gRNA is working, or start with only 1 gRNA at a time? Options: 1 gRNA + Cas9 (1...
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I regularly have low DNA concentrations on the vetigastropod tissues I extract. I have tried fresh tissue and older/museum tissues. We use the Thermo Scientific GeneJET Genomic DNA Purification...
09 April 2024 3,655 4 View
I am running ELISA for different biomarkers against meningitis. I need to know how to calculate cutoff values for it in ELISA. There is no negative control or positive control for these kits,...
08 April 2024 3,160 1 View
Hi, I have recently been working on RNA purification from RNA-binding proteins. I used a protein-specific antibody to immunoprecipitate the protein RNA complex and then extracted RNA from the...
02 April 2024 5,173 1 View
I have a plasmid with kanamycine antibiotic resistant gene and Bar as a marker gene. I need to transfer this plasmid into AGL-1 strain of agrobacterium tumefaciens. I faced a problem when I follow...
27 March 2024 3,944 4 View
Recently, people working in faunal research have practice to photomontage when identifying a species (in most cases they are not professional taxon experts), and they make a mirror image of one...
22 March 2024 6,738 8 View
The samples are 250 μl serum mixed with 750 μl Trizol Isolation by (Chloroform Method ) Homogenize the sample Add 200 μl Chloroform and mix well then incubate on ice for 15 min, then centrifuge...
20 March 2024 6,838 3 View
Hello everyone! I want to know about research square.Is it okay to submit manuscript to reseach sqaure before submitting to a journal. Actually I have no idea about research square
18 March 2024 2,710 3 View
Hello. I'm making non-denaturing PAGE of DNA fragments for analysis of polymorphisms, but I'm getting wavy bands. If somebody can help me, I will be very greatful. First of all, here's my recipe...
16 March 2024 7,739 1 View
What is genetic restoration of endangered species and why species and genetic diversity are each important for an ecosystem?
13 March 2024 5,895 2 View
I have purchased 'Casein fluorescein isothiocyanate from bovine milk, Type III'. However, I cannot find any literature that defines the Type III caseins. The product documents also do not provide...
11 March 2024 6,854 3 View
Hello everyone, Can anyone please let me know how can I extract DNA of Cyanobacteria (Gram Positive bacteria) in on site?
10 March 2024 2,853 1 View
What is the best way to find a bunch of email addresses related to academics working on "Plant Endophytes"?
08 March 2024 5,441 1 View
Greetings to all the professors, Scientists, lectures, can anyone suggest a research paper on cleaving complementary sequences from oligonucleotide DNA- probes without disturbing the conjugation...
07 March 2024 3,989 0 View
Hi! I used the NEB PNGase-F denaturing protocol to denature some lysates and ran them on Western. I usually load 20µg of protein on western; so I did that with my un-denatured lysate control as...
29 February 2024 3,667 1 View
We would like to perform Methylation-Specific PCR using TaqMan primers and probes. With this method, we wanted to determine the DNA methylation of the promoters of the A (PGRA) and B (PGRB)...
29 February 2024 7,452 0 View
Hi, these eggs were found in the fecal samples of small wild mice. So far we have not been able to identify them. Can you help us? Thank you
28 February 2024 8,941 0 View
Hello, I am planning an experiment to detect human antigen-specific TH2 T cells and would like you to learn from your previous experience. By using flow and ICS, in a time frame of 6h or overnight...
26 February 2024 9,145 2 View
Hello everyone, I need a citrate buffer with an optimal pH of 4.8 for the enzymes I'm working with. I'm using citric acid monohydrate (molecular weight: 210.14 g/mol) and adjusting the pH with...
26 February 2024 2,338 10 View
What should be the temperature for PCR machine lid during ligation overnight at 16c? Will the usual lid temperature at 105c affect the ligase performance?
21 February 2024 7,814 3 View
Hi, My project is about co-culture in which my producer strain will be in the biofilm state. I can't find enough literature on the same where they have used biofilms in fermentation for the...
21 February 2024 2,855 2 View
Hi, I'm just enquiring what seeding density people use for LHCN-M2 myoblasts and myotubes? Also how long do they leave RNAiMAX in the media for transfection before replacing with fresh media?
20 February 2024 2,653 0 View