PCR, Cloning, Restriction Digestion, Ligation, Transformation, Plasmid et al | Contact experts in Molecular Biology to get answers
1,965 views 3,277 posts
Questions related to Molecular Biology
Greetings to all the professors, Scientists, lectures, can anyone suggest a research paper on cleaving complementary sequences from oligonucleotide DNA- probes without disturbing the conjugation...
07 March 2024 3,934 0 View
Hi! I used the NEB PNGase-F denaturing protocol to denature some lysates and ran them on Western. I usually load 20µg of protein on western; so I did that with my un-denatured lysate control as...
29 February 2024 3,580 1 View
We would like to perform Methylation-Specific PCR using TaqMan primers and probes. With this method, we wanted to determine the DNA methylation of the promoters of the A (PGRA) and B (PGRB)...
29 February 2024 7,387 0 View
Hi, these eggs were found in the fecal samples of small wild mice. So far we have not been able to identify them. Can you help us? Thank you
28 February 2024 8,854 0 View
Hello, I am planning an experiment to detect human antigen-specific TH2 T cells and would like you to learn from your previous experience. By using flow and ICS, in a time frame of 6h or overnight...
26 February 2024 9,055 2 View
Hello everyone, I need a citrate buffer with an optimal pH of 4.8 for the enzymes I'm working with. I'm using citric acid monohydrate (molecular weight: 210.14 g/mol) and adjusting the pH with...
26 February 2024 2,248 10 View
What should be the temperature for PCR machine lid during ligation overnight at 16c? Will the usual lid temperature at 105c affect the ligase performance?
21 February 2024 7,732 3 View
Hi, My project is about co-culture in which my producer strain will be in the biofilm state. I can't find enough literature on the same where they have used biofilms in fermentation for the...
21 February 2024 2,796 2 View
Hi, I'm just enquiring what seeding density people use for LHCN-M2 myoblasts and myotubes? Also how long do they leave RNAiMAX in the media for transfection before replacing with fresh media?
20 February 2024 2,595 0 View
I work with cell culture of keratinocytes and from the second passage onwards I have to reduce the amount of calcium in the culture medium. Does anyone have a protocol on how to use the Chelex 100...
12 February 2024 9,387 1 View
I’m currently working on the synthesis of aptamers through SELEX and the technique I’m using for converting dsDNA to ssDNA is asymmetric PCR, however when performing PAGE Denaturing...
12 February 2024 1,089 3 View
Hi Is the silkworm considered a worm? In the event that it is transported from the country of origin to another country due to the absence of biological enemies, for example? In the event of the...
08 February 2024 6,577 1 View
I completed the experiment for one of our colaborators (I measured inhibition of an enzyme by 3 compounds). Unfortunatelly compounds were not active in the range of dilutions that I had used (up...
07 February 2024 3,510 2 View
Is It more cost effective to analyze multiple genes using TaqMan versus traditional qRT-PCR? What other main advantages and disadvantages exist for each method? I’m trying to analyze several...
06 February 2024 4,438 4 View
I extracted my DNA samples and ran gel electrophoresis on my templates. Now, I would like to collect the DNA shown in bands on the gel. How do I go about this, please?
03 February 2024 4,604 1 View
Agglutination of RBCs is normally caused by IgM antibodies. Is there any case in which IgG antibody caused the agglutination of RBCs?
02 February 2024 3,528 2 View
Hello Fellow researchers ! I am doing RLM 5'RACE to validate mirRNA cleavage on it's target genes using GeneRacer kit (Thermo Fisher Scientific). After when purified mRNA using Dynabeads where i...
01 February 2024 6,213 3 View
I'm doing this since the ordered antibodies were not received yet?
29 January 2024 6,523 5 View
Hi: I am performing immunofluorescence staining on 100 um 4%PFA fixed pancreas sections using our self-made polyclonal primary antibody. Tissue was gradient dehydrated in methanol diluted in 0.2%...
29 January 2024 842 3 View
actinomycetes isolates molecular identification
29 January 2024 3,571 0 View
Dear all, I've been trying for a long time to isolate pure chloroplasts from a few non-model plant species to obtain pure plastidial RNA. For example, I'm working with a fern (Adiantum capillus...
25 January 2024 5,808 0 View
I am using PCR Instrument called SLAN from HONGSHI. Any clue?
24 January 2024 9,680 7 View
Hello, The biofilm that I am culturing from this strain is too flimsy, such as when I wash the biofilm with PBS it breaks and resuspends even at a very gentle wash. I have been growing this...
19 January 2024 7,119 3 View
Carbon balance
05 January 2024 2,762 0 View