Can i change buffer pH without changing precast gel pH?

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How Can I Run RNA+protein Sample in Gel?

I am trying to do an EMSA with unlabeled RNA an some proteins. RNA is about 700nt long in-vitro transcribed and the 2 protein are about 30 and 60 kDa with PIs 6-6.5. One of the proteins is the...

03 September 2020 5,989 6 View

Do i need to learn programming for end-user bioinformatics?

I'm a molecular biologist, and i have a few projects coming up in transcriptomes and small RNA analysis. Can i get by without knowing any programming using user-friendly software such an Geneious...

10 May 2020 7,868 1 View

Anti-peptide polyclonal antibody Specificity?

Hello, I am designing to produce antibodies against 15aa peptides, these antibodies will be used for detection of a pathogen in plant. The problem is when i blast these 15aa sequences there are...

12 February 2018 6,661 3 View

Having Trouble expressing membrane Protein. Toxicity?

Hey, So i have a 39kDa Membrane Protein, cloned into pRSET C and transformed into C43, sequenced to makes sure everything is there and made a glycerol stock of. From that glycerol stock i start a...

15 December 2017 7,024 3 View

Am i supposed to see membrane protein band in sds page of cell lysate?

If i Lyse cell overexpressing a membrane protein with sonication in 20mM NaH2PO4 and 500mM Nacl, firstly can i load it as it is in sds page or do i have to add SDS loading buffer? Either way ,...

16 November 2017 9,947 8 View

Double digest vector self ligating?

So, i am double digesting pRSET A vector and an insert with BamHI and PstI ... running a gel and purifying the respective bands.... After ligation and transformation , and running PCR on the...

05 October 2017 3,661 3 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

Protein-aptamer gel electrophoresis help, please??

Hi, I have problems with running gel electrophoresis. I have tried agarose gel electrophoresis and native PAGE. I have two proteins, which have molecular weights of ~30kDa and ~180kDa and two...

03 March 2021 4,275 4 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

Does the pressure(-600 mmHg) affects pH?

When I conducted the degassing experiment using decompression under anaerobic condition(and I used ferrous based chelating agent), the oxidized ferric were observerd. So, could you tell me the...

03 March 2021 4,892 5 View