Hello,
I have been seeing a strange contamination in my cells I have not been able to solve. It is Dictyostelium cell culture and therefore anti-fungal cannot be used. Cells have been cultured in amp, strep, and kanamycin. Cells grow in both suspension and adherent culture. No contaminants are seen until the cells are moved from adherent to suspension culture. We will see these in the culture and the media will turn reddish as if its dissolving (there is no phenol red in media). Has anyone ever seen it in cell cultures before?
Crystals are sharp, straight structures. This looks more biological, or could be some aggregate from the medium. What medium are you using? Have you tried filtering it (0.2 µm filter) before adding it to the cells? Actually, I remember seeing such artifacts in the past and I didn't worry unless they were growing in size or moving.
I have a similar problem. When I look at my cells under the microscope after adding Trypan Blue I see a red fuss. My cells have 100% viability, yet they have stopped dividing. I am not sure if it is because of the media. Please let me know what did work for you. Here is a picture of my contamination.
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