CRISPR sgRNA contains sequences for target (20nt), Cas9 handle (42nt) and S. pyogenes Terminator (41nt). Is there a need for host specific Terminator?

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Mehrdad Mohebi Shahpour Abadi

Hi Sandeep K. Panda,

The answer to the question is in the article below.

Article Development of a versatile Cas9-driven subpopulation-selecti...

Sandeep K. Panda

Thank you. I have read the paper. It doesn't explicitly tell what terminator was used, rather cited another paper from Prof. Zhang.

From other papers it is understandable that they aren't using any host specific terminator.

Pei-Zheng Zheng

https://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=&cad=rja&uact=8&ved=2ahUKEwiom8v13KrwAhUBzTgGHbJ2DboQFjABegQIAxAD&url=https%3A%2F%2Fwww.researchgate.net%2Ftopic%2FCRISPR&usg=AOvVaw2b3KouSi7ex0je4qKp4s0t