I am creating CRISPRi tool for Lactococcus lactis. Do I need to add an extra Lactococcus Terminator sequence at the end of the sgRNA or is the S. pyogenes Terminator enough?
Hi Sandeep K. Panda,
The answer to the question is in the article below.
Article Development of a versatile Cas9-driven subpopulation-selecti...
Mehrdad Mohebi Shahpour Abadi
Thank you. I have read the paper. It doesn't explicitly tell what terminator was used, rather cited another paper from Prof. Zhang.
From other papers it is understandable that they aren't using any host specific terminator.
https://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=&cad=rja&uact=8&ved=2ahUKEwiom8v13KrwAhUBzTgGHbJ2DboQFjABegQIAxAD&url=https%3A%2F%2Fwww.researchgate.net%2Ftopic%2FCRISPR&usg=AOvVaw2b3KouSi7ex0je4qKp4s0t
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