I am using QIAGEN's AllPrep DNA/RNA/miRNA Universal Kit. The handbook doesn’t mention the possibility of additional DNase digestion post-elution, i.e. directly in the RNA solution.
Can this additional in-solution DNase digestion (and/or subsequent DNase heat (65-75C) inactivation) damage miRNA or lower yield and so should one only use in-column DNase digestion as per protocol?
Craig Silver
Heat can (and will) speed up the degradation of RNA. I suspect that is definitely the cause of lower than expected yields.
0 votes
0 thanks
Lora Benoit
in the past, the only dnase 1 that worked for me to clean up rna was from Ambion using a kill step.. We lost rna using the qiagen kits.
0 votes
0 thanks
Kazi Tawsif Ahmed
Dear Maxim
definitely heating the mixture will speed up the process of rna degradation.
0 votes
0 thanks
- Badges
- Science topic
- Similar topics
- Biological Science
- Biochemistry
- Biomolecules
- Nucleic Acids
- RNA
- Small RNA
- microRNA
More Maxim Dukov's questions See All
Similar questions and discussions