What is the name of doctor, professor B. Romeis?

In one paper I found a mention of professor B. Romeis, who wrote a book on "Mikroskopische Technik" (1936). I need to mention him in my paper, but I cannot find his full name anywhere. Even in...

13 January 2021 7,402 3 View

How to improve Desmond MD/metadynamics calculations performance?

Which program parameters is useful to improve calculations performance (ns/day) of MD simulations without simulation quality loss?

28 June 2020 4,840 2 View

Does it make sense to model mass spectrometric fragmentation by DFT?

Hello. I have met a significant number of publications on the study of the laws of mass fragmentation using DFT. Researchers are building huge schemes where they show in which direction the decay...

05 February 2020 6,252 3 View

How to solve problem with momentum overflow in desmond metadynamics simulations?

During metadynamics calculation, Desmond gives error: -- Allowed momentum exceeded on 2 particles. 7187: force = (-3.0580500e+04 4.4492168e+04 -7.2957642e+03) momentum = (-2.9018593e+01...

03 September 2019 8,099 2 View

Is there a formula to calculate minimum required coverage for NGS given a certain variant allele frequency and sequencing error?

I'm looking for a formula that calculates minimum coverage needed to call a variant allele with a certain condifence level, given expected variant allele frequency (VAF) (or percentage of mutants...

05 April 2018 9,336 1 View

Any recommendations as to how many bytes/reads/bp per sample are needed for shotgun/16S metagenome sequencing with genus-deep taxonomic profiling ?

20 March 2018 7,619 4 View

How much Resazurin powder should be added to 10 ml of culture medium to prepare a solution for a mammalian cells viability assay?

I have Resazurin powder from Sigma but I don't know which concentration must have a stock solution or a final solution for viability test. Please, help me.

05 December 2017 7,371 2 View

Stat analysis on the sequence of events

Hi all, I need an advise on the correct statistical approach for a sequence of events. 1. I have a data collected from a group of individuals at baseline (BL) and follow-up (FU). 2. There are...

04 June 2017 8,081 5 View

Does culturing of ASC lead to a 100% pure collection of ASC? Or does it contain other adherent cell types?

Dear all, I am starting an experiment with adipose derived stem cells (ASC), but I would like some help or confirmation for the following issue. Background: I am harvesting murine fat from the...

10 July 2016 3,373 17 View

No title

18 February 2016 1,339 2 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

MiRNA qpcr problem?

Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.

28 February 2021 5,008 4 View

For modelling epoxy-based matrices (in composites), which model in ABAQUS is the most suitable, Concrete damaged plasticity or Drucker-Prager model?

I am working on developing a micromechanical FE model for predicting kink-band formation in UD composites. To model matrix plasticity, which model, out of Concrete Damaged Plasticity and...

28 February 2021 5,415 1 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Why is the efficiency of viral mRNA electroporation in PBMC low?

Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...

27 February 2021 9,233 3 View

Can MicroRNA cross the blood brain barrier?

In diseased conditions, many genes and gene products are visible in the bloodstream. So my question is whether we can find traces of microRNA which is involved in disease and disorder in blood serum?

24 February 2021 7,064 3 View