Recommendations for Books and Articles on Transcriptomics and RNA-Seq?

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What is the difference between OTU and ASV analysis?

For microbiome data analysis

13 July 2024 4,542 2 View

How to calculate the shading losses of a pv panel?

I have set up a small pv farm on a rooftop of a factory using a software called pvsol. The pv farm consists of 100 modules arranged in a specific arrangement. I've got their rated output power and...

22 March 2024 5,945 1 View

How Can we handle min max problem with multi-objective optimization using Gray relation analysis !?

In the case of three output responses, two of them will be at the desired level's minimum, and the third will be at its maximum. Assume two responses surface roughness and roundness error , while...

30 September 2023 1,755 0 View

Can we carry out gene ontology using a redefied data (from NCBI) for my organism??

I mean if I can use the transcriptome data from NCBI (as example for rats) to do gene otology or not

15 September 2023 213 1 View

What is this error in General Linear Model in Minitab?

Hi every one! Do you know that what is this error in General Linear Model in Minitab 20? ''The following terms cannot be estimated and were removed: A*B, A*C, B*C'' Why sometimes Minitab can't...

13 August 2023 5,774 3 View

What is the meaning of “number of atoms” on the material project website?

Could anybody explain me the meaning of the data point “number of atoms” that is shown for any material on the material project website? I thought that it was supossed to tell the number of atoms...

09 August 2023 7,921 2 View

How to obtain the phase change using the IQ data received from the ADC ?

I receive the received signal using an unmodulated continuous wave radar sensor and I'm struggling to obtain the relative distance of the object. I receive the IQ data in milli-volts and use the...

20 May 2023 301 1 View

Anyone knows the projections for hydrogen storage technologies?

I search for projections (current status) for different hydrogen storage technologies achievements in gravimetric and volumetric storage densities, and costs to compare with DOE targets ?

02 May 2023 8,930 2 View

How can I find the distance of a target from a CW radar sensor ?

I obtained the IQ signal samples and stored each in a separate array, then I applied the following formula to obtain the phase difference delta_phase = arctan2(Q_samples(t)/I_samples(t)). Then...

30 April 2023 2,012 9 View

What is PatchDock's unit measurement?

I'm whiting a paper about molecular docking, to calculate receptor and ligand interaction i used patchdock server (https://bioinfo3d.cs.tau.ac.il/PatchDock/patchdock.html). My question is what...

13 February 2023 6,769 1 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Strugglling with m6A dot blot any suugesstion ?

I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...

10 August 2024 8,539 5 View

RNA Extraction Using Hot Borate Method No Longer Working?

I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...

08 August 2024 9,882 2 View

Does Anyone have expertise in in vitro transcription and RNA pull down assay?

I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.

07 August 2024 6,622 1 View

E.coli contamination in human RNA seq data ?

Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...

06 August 2024 807 3 View

RNA later for the preservation of RNA in fecal samples at room temperature for one day (37°C)?

I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...

06 August 2024 1,367 3 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 501 2 View

What is the acceptable p-value cutoff for GO enrichment analysis ?

I have an RNA-seq data that I have analysed using Limma-voom and have extracted the gene IDs, log2FC and the p-values. At p value < 0.05, I have over 10,000 DEGs, however, when I run the GO...

31 July 2024 225 2 View

Inquiry on Maximum Nucleic Acid Volume for 2.5 mL Liposome Solution?

I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...

30 July 2024 6,420 1 View

How to prediction 2D to 3D aptamer?

Hello, I have a little problem. Well, right now I only have the DNA aptamer sequence. I went to see the 2D model form with DNA folding. After that, I used RNA Composer to simulate in 3D and used...

30 July 2024 6,794 0 View

Frederic Lepretre

Hi Seif

you should try YouTube, it will offer you ideas.

fred

Joachim R. Grün

Hello Seif,

I have been working on gene expression profiling for many years now. We have detected several biomarkers with our methods and, up to now, 4348 citations of our 231 Research articles. Try https://www.researchgate.net/profile/Joachim-Gruen/research.

Seif Hundam

Hello Dr. Grün,

Thank you for reaching out and sharing your experience with gene expression profiling. It’s impressive to see the extent of your work and the impact it has had with such a high number of citations across your publications. I appreciate the link to your ResearchGate profile, and I look forward to exploring your research further.