How long is it possible to store the bacterial culture at 4C?
Some bacterial strains can be stored for up to 1 year at 4°C in agar stab cultures, which are especially useful for transporting samples to other research facilities.
https://www.thermofisher.com/in/en/home/industrial/microbiology/microbiology-learning-center/storing-bacterial-samples-optimal-viability.html
Some bacterial strains can be stored for up to 1 year at 4°C in agar stab cultures, which are especially useful for transporting samples to other research facilities.
https://www.thermofisher.com/in/en/home/industrial/microbiology/microbiology-learning-center/storing-bacterial-samples-optimal-viability.html
For bacterial cultures, generally we can store them for few days at 4°C depending on the type of bacteria.
It all depends upon what you intend to do with them. If you are just storing them and then plan to streak them out again later, strains should last for months at 4C. However if you want to actually do experiments on the cells you are storing, then probably 24 hours at most before you start having physiological changes.
Note that with E. coli strains if the strain is a recA- strain (like many cloning strains), then their lifespan is MUCH shorter and you will start getting significant cell death in a week or two at 4C.
Please have a look at the following link:
http://www.protocol-online.org/biology-forums/posts/37544.html
It depends on what type of bacteria you are culturing and whether they are in liquid culture or on agar plates. With E. coli, agar plates sealed in parafilm and kept in the fridge are viable for a few weeks. If you are looking to preserve a culture long-term, especially if the bacteria are carrying a particular plasmid you want them to keep, it is best to maintain them as a 25% glycerol stock at -80C.
I do agree with Prof Arvind Singh . Some bacterial strains can be stored for up to 1 year at 4°C in agar stab cultures.
Storing Bacterial Samples for Optimal Viability | Thermo Fisher ...
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Thualfakar Hayder Hasan Abusaiba
As explained by Arvind Singh !! In my experience,yes, it is right that some bacteria can stay alive at 4°C over a year. Provided you must know that how to perform this exercise !! I had revived my bacterial strain after a year stored at four degree.
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