Is this an acceptable method for determining the minimum inhibitory concentration of plant extract using macrobroth dilution technique?

17 August 2020 3,695 27 View

Why do 293T cells starts dying after lentiviral transfection?

I have a lentiviral transfection protocol on 293T cells that has been working for years, but for the past few months, my 293T cells starts de-attaching from the plate. I finally figured out it was...

16 August 2020 6,647 3 View

Is this formula correct for measuring growth inhibitory indices for synergy index?

GIIs= ZDI in combination / total of ZDIs of the two agents in single action in order to corroborate the synergistic activity of the antibiotic in combination with the plant extracts, and synergy...

23 May 2020 9,095 5 View

What is the optimum time for agar pre-diffusion of plant extract in disk diffusion method?

27 April 2020 8,665 26 View

Is this a good method for plant extraction for antimicrobial activity?

25 April 2020 9,907 35 View

Does anti-serum vaccine works against the novel coronavirus?

The anti-serum will consist of antibodies extracted from those who already recovered from the infection.

22 March 2020 5,331 11 View

How to prepare a 50% DMSO plant extract?

How to prepare 50% DMSO from 100% DMSO? What is the solution should I use to dilute it? I have water and chloroform. The plant material is initially extracted in chloroform. Need to...

16 December 2019 5,687 8 View

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30 August 2019 610 9 View

Is there a good chance of increasing IELTS band score for writing & speaking from 5.5 to 6 after a remark?

What are the chances of increasing IELTS band score for writing & speaking by 0.5 after a remark (EOR)?

27 May 2019 4,147 11 View

Which plant extract is better for antimicrobial sensitivity testing (disk diffusion assay)? centrifuged or non-centrifuged extract?

Which plant extract is better for antimicrobial sensitivity testing (disk diffusion assay)? centrifuged or non-centrifuged extract?

23 February 2019 2,103 8 View

How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View

What does Servant Leadership mean today and in the future? What are the advantages and disadvantages?

In current time of many changes in business and world of work, a lot is said and reported about the importance, skills and benefits of leadership. Are soft skills becoming more and more important?...

02 March 2021 8,149 3 View

Which SAR dataset to use for generating coherency matrix and performing target decomposition (Description) ?

Working on chandrayaan-2 DFSAR data, there are three datasets available: 1) Slant range image data product: The slant range complex image file. Each pixel is represented by two 4-byte floating...

02 March 2021 8,481 3 View

How to grow E.coli on the low salt LB agar plate with 50 or 100ug/ml of Blasticidin?

Hello, I would like to ask how to grow E.coli which resists 50 or 100ug/ml of Blasticidin. I have tried many methods by using low salt Luria-Bertain(LB) agar plate. However, no colonies grow. Let...

01 March 2021 2,027 2 View

How UAV or drone can be beneficial to better estimate crop yield?

As we know, crop yield estimation has been done traditionally with farmer's expert knowledge, deductive sampling in representative areas, and remote sensing with satellite imagery. Farmer's Expert...

01 March 2021 3,468 3 View

Which bacteria are suitable for quorum sensing screening?

I want to find a quorum sensing inhibitor from plant extracts. Klebsiella pneumoniae, Moraxella catarrhalis, Staphylococcus aureus, and Streptococcus pneumoniae. I looked for these strains...

01 March 2021 7,675 3 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

Can bacterial suspension be pipetted directly onto petri dish and agar poured onto it?

Hi, recently I encountered a protocol for plating which was not the usual method I have been practicing (pour LB agar onto plate, allow it to solidify, pipette bacterial suspension onto plate,...

28 February 2021 7,292 3 View

What is the exact mechanism of the antimicrobial effect of local anesthetics?

The antimicrobial effect of local anesthetics has been known for more than 50 years, but it still seems to have an unclear mechanism of action. which bacteria? which concentration? etc etc. ....

27 February 2021 4,450 2 View