Can I use Tween 20 instead of Tween 80 for Spore Suspension?

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Can we mark 'EFL Learners shifting from general digital to AI technologies' as technological transition?

After COVID-19 it has seen that EFL learners technological affiliation has raised. In addition, in the post-COVID period learners started to engage AI technologies like ChatGPT while learning...

08 August 2024 8,964 4 View

How to generate a citation of my paper from ResearchGate?

How we can cite the papers from ResearchGate. I am trying to create citations for this article, Quantum Machine Learning Algorithms for Optimization Problems: Theory, Implementation, and...

08 August 2024 6,690 3 View

Does Anyone have expertise in in vitro transcription and RNA pull down assay?

I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.

07 August 2024 6,622 1 View

How to fix background error in rietveld refinement of one XRD peak using GSAS-II?

I want to refine one XRD peak of my in-situ xrd but the background is never working good which ultimately fails the refinement. How to refine and adjust the background using GSAS-II

05 August 2024 5,291 2 View

How can I add own Henry coefficients in Aspen Plus?

Hi, i would like to simulate an absorption process in Aspen Plus. I want to use the NRTL model und would like to add some individual Henry coefficients. Is that possible and how?

05 August 2024 2,333 2 View

Why might the impedance values for DI water and 0.1X PBS buffer solution exhibit a decreasing and increasing trend, respectively over time (HP 4194A)?

Hello everyone, I'm encountering an issue with my electrochemical impedance spectroscopy (EIS) measurements and would appreciate some insights. Experimental Setup: Electrodes: Gold interdigitated...

05 August 2024 3,783 2 View

Can usage of AI tools like chat GPT in research work is recommendable ?

AI tools like ChatGPT can enhance research work significantly when used responsibly and in conjunction with thorough human oversight.

05 August 2024 1,842 3 View

Usage of internal standards in LC-MS/MS analysis?

Have you ever seen a LC-MS/MS method uses both internal standards and external standards (in matrix matching purpose) but the concentrations of internal standards are outside the calibration curve...

05 August 2024 3,084 6 View

ANY free software for reconstructing neurons in the microscopic image?

Hi everyone, I am working on brain slices for visualizing a protein in the soma and dendrites, using a fluorescence tag. However, I need a tool (not paid) for reconstruction of the whole neuron,...

04 August 2024 4,725 2 View

How effective is the Citi Bloc standard basket in enhancing the accuracy and comparability of international construction cost assessments?

Citi BLOC Standard Basket Definitions: A standardized unit representing a fixed basket of construction materials, labor, and equipment costs priced in various cities. Purpose: To create a common...

04 August 2024 8,997 1 View

How can I prepare virus for a TEM or SEM imaging?

I have virus (viral hemorrhagic septicemia virus) in suspension and the experiment will not involve cells. What level of TCID50 is preferred?

11 August 2024 3,115 1 View

CHO-K1 suspension adaptation protocol?

I am trying to adapt adherent CHO-K1 cells in F12K+10%FBS to suspension culture in only F12K, but in vain. I have followed Mirus bio as well as Thermo protocols, please suggest any alternative....

31 July 2024 3,756 0 View

How do I successfully culture primary human leukocyte from TLRE filters and improve viability of the cells in suspension?

I am currently culturing leukocytes from TLRE filters from our blood bank. I have been struggling to culture these cells, as well as improve their viability. I was wondering if anyone would be...

16 June 2024 3,752 0 View

How to visualize a cell palett of a small cell amount?

Have you ever seen a small number of cells, 50-100 thousand, after centrifugation as a dry pellet in an Eppendorf tube without a microscope? Are there methods to potentially stain the cells in the...

09 June 2024 6,311 0 View

Can you move immunofluorescence suspension sample?

I was wondering if you can move immunofluorescence ready sample that is in the form of suspension on 96-well plate to another well plate. Is that possible?

14 May 2024 596 1 View

Has anyone attempted to adapt UMNSAH/DF-1 (Chicken Fibroblast cells) into suspension cells?

Anyone tried adapting UMNSAH/DF-1 (Chicken Fibroblast cells) from adherent to suspension cells. Currently we are using Xerumfree TnC bio serum alternative with DMEM. The cells are not adapting...

05 May 2024 3,316 1 View

How to Isolate Lipase producing fungi from Shea butter and Ghee?

I'm unable to get a clear zone of hydrolysis after using Tween 80 with phenol red agar This was after we already used Tween 80 as enrichment media. We used Shea butter and Ghee that have been...

05 May 2024 8,272 0 View

Can we use cefotaxim antibiotic in THP-1 cell line?

i am doing THP-1 suspension culture and using cefotaxim as a antibiotic but after 4 passag my cell count was decline, so is it becuase cefotaxim my cell is decline

01 May 2024 9,779 3 View

How to do efficient manual counting of cells?

I am struggling with manual counting using neubauer chamber. We have automated counter as well. Manual counting shows lesser cell density while automated ones show more. Mostly people say manual...

30 April 2024 278 0 View

BCA assay and the reported protein concentration?

In the BCA assay, are there any differences between the reported protein concentration directly from the cell suspension and from the protein extracted solution of the cells by RIPA buffer?

30 March 2024 8,359 0 View