What could be some possible experiments/fields of research with only 25 S.aureus strains from bovine mastitis?

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Dear Scholars, do you have the latest data (1982 to 2019/2020) of ICRG (International Country Risk Guide)?

Dear Respected Scholars, I am in need of the latest data set (1982 to 2019/20) of ICRG. I would be grateful If you share those data with me. My email address: [email protected],...

13 February 2021 2,631 1 View

What is the best analytical software for XRD data?

How can I determine any phase from the powder XRD data and how can I determine the morphous or amorphous state of the sample from XRD data?

09 February 2021 7,867 5 View

Will the wider bandgap be at the core or outer layer of radial sc?

I am simulating a radial p+AlGaAs-pGaAs- n+GaAs Solar cell. AlGaAs has the wider band gap. I want to know if and how it matters whether tha AlGaAs is at the core or the outside. I know in tandem...

06 November 2020 9,293 4 View

What if I can't find k values for n,k files in Atlas, Silvaco ?

Hello, I am looking for simulating GaAs(x)N(1-x) Material is Atlas, But I am not able to find the k values to give in the n,k index files, but I have the data for n. What should I do?

30 July 2020 7,912 2 View

Can anybody please send me MatLab code for oppotion based PSO/BBO?

Opposition based learning

05 April 2020 659 4 View

How can I measure the amount of essential oil and tannic acid uptake in nanoparticles like chitosan, silica by Thermogravimetric analysis (TGA)?

I want to measure the Essential oil and tannic acid uptake in nanoparticles like Chitosan, silica by TGA. Can anyone provide me a protocol for that?

14 February 2020 5,749 2 View

What is Bait/Hit role type in Biogrid gene interactors table?

Please find below the interactors table for gene GNB2 from BIOGrid. The Role column provides a sense of directionality to the interactor mentioned in column 1. Can anyone kindly clarify the...

29 October 2019 4,089 1 View

What are the gene probes in Human Genome U133A Affymetrix platform especially?

I have come across the dataset GSE15852 which is an expression dataset from human breast tumors and their paired normal tissues. The microarray study was done on platform GPL96 [HG-U133A]. I...

25 September 2019 5,277 5 View

Is there a way to know which rules are fired in FIS designed in MATLAB?

I was looking for a method such that the firing strength of rules in FIS is known. Basically I want to know about the variety in data inputs I have. So if similar data inputs I have every time,...

16 September 2019 4,567 2 View

How can determine the Quantity of liquid desiccant for air dehumidification system? Required Temperature for Regeneration??

Liquid Desiccant air dehumidification System Design with regeneration

24 June 2019 2,341 1 View

Why does intensity drops as concentration increases?

Is there any reason for decrease in fluorescence emission intensity of HSA,BSA when increase the concentration ?

28 February 2021 3,653 4 View

Antibody anti-IgM Bovin conjugated Biotin ?

Hi everybody, Can anyone please suggest me an antibody anti-IgM Bovin conjugated Biotin ? I would like to use it in LUMINEX with a streptavidin conjugated phycoerythrin reporter. Thanks in...

25 February 2021 9,659 2 View

Questio regarding micro/nano particles fabrication ?

I will start to fabricate micro/nanoparticles for growth factor release and I check out a procedure quite nice, however, it is lacking some details and since I don't have much knowledge doing this...

20 February 2021 199 2 View

How to successfully revive cells stored at -80 degree C for a year?

I am working on C6 cells along with other neuronal cell lines. We have preserved the cells in 90%FBS and 10% DMSO solution. We are reviving the cells after a year and cells are either not binding...

18 February 2021 4,499 1 View

Why does flow deformation occur at the test line and control line on nitrocellulose membranes (LFIA)?

I use 1% BSA in 10 mM phosphate buffer (pH 7.4) solution when striping my nitrocellulose membranes with antigens and antibodies. However, I always observe deformed flow when my sample or running...

16 February 2021 4,224 3 View

What is a suitable method to freeze feeders-free, serum free episomal hiPSC?

Hi everyone, I need your professional advice: I started growing episomal iPSC (commercial cell line). I grow them in StemFlex medium. What would you recommend as the most effective freezing...

07 February 2021 8,591 4 View

Endothelial Functional Assays & Conditions?

Hi everyone, I would greatly appreciate it if someone can shed light on certain endothelial functional assays and their conditions. I often notice that protocols are run with serum-free media, or...

05 February 2021 7,276 1 View

Moving bacteria-like organisms in my HEK293T cell culture?

Hi everyone, I have been culturing HEK293T cells that have been engineered to overexpress the mechanosensitive protein Piezo1. However, I am finding it very difficult to keep them in culture -...

02 February 2021 7,892 4 View

Freeze/Thaw of LS174t cells?

Hello World, I have been working with LS174t. Complete Growth Media- 10% FBS/EMEM. ATCC says to freeze with 5% DMSO/CGM. when I defrost my vials, they are not attaching to flask. vials put into...

01 February 2021 8,723 2 View

What is the difference between blocking buffer tbs vs. pbs and which should I use?

Hi everybody! I am doing western blots on bovine mammary epithelial cells to determine casein. We have two types of blocking buffer in the lab. PBS:...

01 February 2021 9,278 5 View

Chinaza Godswill Awuchi

Kindly check https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6147393/

Also check https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7277341/

Karima Galal Abdel hameed

Dear Satwik Majumder

plz check. The attached article

Nawel Zaatout

Ive published an article with only 9 isolates, you can search for biofilm formation genes and MSCRAMMs genes.