Which is a lucid standard comprehensive book to guide on psychological scale construction and its standardization?

02 March 2021 7,082 3 View

How to calculate enthalpy of formation of Polymers taking into account the terminal end groups?

I am trying to find methods for theoretical calculation of enthalpy of formation of Polymers. till now I have found the oligomeric method (Gaussian) which uses the extrapolation of results from...

05 February 2021 9,707 4 View

Can any tell me or suggest any research article or review about the concentration of peptide dissolve in system water and given to Adult Zebrafish?

Hello, everyone! I have chemically synthesized antioxidant peptide isolated from the plant. I have to validate its bioactivities in Adult zebrafish. I have to administer this peptide to zebra...

01 February 2021 5,809 3 View

How can we polymerize carbon to form a layer? Any easy method is there?

I want to polymerize the carbon to make a thin sheet. Is there any easy method to polymerize it?

10 December 2020 1,762 4 View

I am designing an UWB antenna..Please guide how to get wideband instead of multiband..?

By slotting patch and ground I am getting multiband not wideband.pls guide

09 November 2020 3,924 4 View

What is the best way to learn materials simulation using MATLAB?

I want to study the electronic, optical or catalytic properties of two-dimensional materials using MATLAB. What is the best way to start learning?

26 October 2020 1,821 8 View

Can I run joint wald test for more than two regressions to interpret whether the intercepts are jointly equal to zero or not?

I have run 10 regressions using same model for a sample by diving the the sample on the basis of some criteria. The problem I am facing is that I can test the intercepts for individual sample...

25 September 2020 6,314 3 View

Is Cronbach's alfa value 0.5 acceptable ?

I am calculating the whistleblower policy disclosure score for 100 Listed firms in India. I took 31 questions under four categories and scored them 0, 1 & 2 on three-level a) 2 points: If...

09 June 2020 7,729 3 View

My Work Is On Information Retrieval Ranking Using Pso+Svm ,Using Trec Dataset Of Q/A ,But Some Good Journals Rejected Commenting Its Basic,Is It?

I did my implementation in python and represented my result score result of answers of the questions.I represented graph for all the evaluation parameters and confusion matrix,why i am not able to...

24 March 2020 4,235 3 View

What are the statistical method or procedure to construct Index?

I am constructing the whistleblower policy Disclosure Index for the top 100 listed companies in India. I have taken 31 attributes/questions/parameters under four major categories i.e. Board...

23 March 2020 6,571 3 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Why do my MCFs not form monolayer?

Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...

27 February 2021 3,904 2 View

Why is the efficiency of viral mRNA electroporation in PBMC low?

Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...

27 February 2021 9,233 3 View