Where is the best site to gating the osteoclast by Flow cytometry?

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Looking for advices and tips to improve the staining and the gating strategy in human blood?

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Sarah R. Amend

Osteoclasts can't be used in FACS because of their morphology and strong adhesion -- the cells are quite large and "sticky," so lifting them will result in shearing.

You are better off using on-plate methods (staining, IF, etc) for culture or IHC for in vivo work.

Claudine Blin-Wakkach

In fact, yes you can analyze osteoclasts by flow cytometry. But you have to work with "small" osteoclasts (