Is it tricky to use MS to sequence a small recombinant portion of salmon IgT?
Hi guys, I have an experiemnt where three groups of fish were used, two of them were treated with two doses of virus, while the thrid is a control group. I want to compare the means of gene...
06 July 2019 3,718 5 View
I am producing a recombinant protein in Rosetta, my protein is fused to MBP+his-tags at the amine terminus. I have solubilized it with sarkosyl. I am using IMAC column to purify my protein and...
02 March 2017 1,285 9 View
is it necessary to remove the sarkosyl- my r-protein is solubilized in sarkosyl- before mice immunization?
02 March 2017 6,722 2 View
I will purify my r-protein (produced in E.coli) and cleave the MBP and His-tag off it and I have heard about the inclination of the r-protein to aggregate/precipitate when the tag cleavage takes...
02 March 2017 5,644 1 View
My r-protein (in e.coli) is only solublized in urea and I want touse it in a downsteam immunization procedure, dentaured protein is not convenient for production of Ab, how to solve this problem ?
02 March 2017 9,202 2 View
I have a question regarding to your Amylose resin kit https://www.neb.com/products/e8021-amylose-resin I read in the protocol...
01 February 2017 9,668 7 View
I am producing my MBP+his-tagged r-protein in Rosetta (75 kDa). I am using the anti his-tag column t purify my protein. I collected the sample flow-through after passing through the columns in...
01 February 2017 3,961 12 View
Hi guys, I want to ask about RNA isolation procedures from fish skin tissue samples? a full protocol would be appreciated.
02 March 2016 5,239 3 View
I have a question regarding the result of MS (protein sequencing). We made the recombinant r- IgT (unique immunoglobulin in fish) in mammalian HEK cells, we want to characterize the r-protein, we...
11 December 2015 6,059 6 View
Do you know If the urea is quite more efficient than SDS as a protein denaturant? I always experience a smear in my SDS-PAGE and I thought Urea may help me in getting rid of this smear.
10 November 2015 4,169 14 View
I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...
07 August 2024 6,034 3 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
Some Staphylococcus aureus strains Inhibit the growth of Mycobacteria in Mueller Hinton Agar medium containing 10% OADC. Do some Staphylococcus aureus strains have in vitro antimycobacterial activity?
29 July 2024 10,023 2 View
I have immunized BalB/C mice with a protein using the intradermal (ID) method with Complete Freund's Adjuvant (CFA) and Incomplete Freund's Adjuvant (IFA), following a 14-day interval and three...
22 July 2024 9,160 2 View
I'm currently using Roche anti-DIG-AKP Fab fragments to perform Southern Blot assays, but this reagent is delayed since november last year. It's anybody facing the same problem? If so, how are you...
15 July 2024 6,668 0 View
What are the opportunities and limitations for using digestate ion different crops and soil typs and how can its application be optimized - I have few of them listed and can be updated in this...
13 July 2024 2,431 2 View
Hi all, I would like to know if it is possible to use Bovine Serum Albumin (BSA) in my blocking solution for immunofluorescence muscle fiber typing. The primary antibodies' host is mouse. The...
07 July 2024 7,967 3 View
My study uses C57BL/6J mice and I am required to store portions of the important organs in neutral buffered formalin (NBF). Could I potentially keep those tissues in the formalin for a few weeks...
06 July 2024 8,360 2 View
I am working on an in-vitro model of hypoxia-reoxygenation (H/R) in cardiac cells (H9c2). Due to the unavailability of a hypoxic incubator, I am exploring alternative methods to create hypoxic...
30 June 2024 1,234 2 View