We are using KLH as an immunostimulant. I am trying to find the right filter size (guessing 20-40 micron range) for filtering the suspension before injecting in vivo to assess immune stimulation. Thank you.
Filter-Ag granules have irregular surface characteristics affording maximum removal of suspended matter throughout the filter bed.
Filter-Ag can be applied to systems designed for either pressure of gravity flow. It typically removes the normal suspended solids down to the 20 – 40 micron ranges. Advantages of Filter-Ag include less pressure loss through a bed of Filter-Ag than most other medias. It is lightweight and requires lower backwash rates.
Filter-Ag has high dirt removal capacity that results in longer filter runs with a substantial saving in labor cost, backwash water and time out service.
Advantages
There is less pressure loss through a bed of Filter-Ag than through most other filter medias
Light weight requires lower backwash rates than those required for other filter medias
High service rates in lower equipment costs and a savings in space
High sediment reduction capacity results in longer filter runs, with a substantial savings in backwash water and time out of service
Reduced shipping cost due to light weight/cu. ft.
Replacement of sand with Filter-Ag in existing installations may increases filter capacity 100% or more. (Caution should be taken upon start-up that the lightweight Filter-Ag is not washed to drain.)
We generally use a .22 micron syringe filtre without any problems, although if you are injecting KLH in Freund's or another oily medium, you might want to go to a larger pore size to limit the amount of force (and contamination risk) needed to get the solution filtred.