can some one please tell me specific markers for Cardiac Fibroblast to confirm the presence of Cardiac Fibroblast?
This paragraph is from the article attached bellow. Maybe it can help you.
"A number of cell surface markers have been identified for FBs and CFBs, but over time their specificity to these cells has been challenged. Vimentin, a protein that is present in the intermediate filaments of FBs, has been the most widely used FB marker – and although it is also expressed in other cell types such as endothelial cells [8] and myoepithelial cells [9], due to morphological differences among these cell types, vimentin remains a reliable marker for identifying FBs [10]. Discoidin domain receptor (DDR) 2 was discovered as a specific marker for CFBs [1,2,11]. DDR1 and DDR2 are collagen receptors [12,13], a family of protein tyrosine kinases involved in a variety of cellular functions such as growth, migration and differentiation [14]. DDR1 is expressed mainly in epithelial cells, whereas DDR2 is expressed in mesenchymal cells [15]. DDR2 was detected in rat and mouse heart [16], and has been considered to be more specific than vimentin for CFBs since it is not expressed in cardiomyocytes or cardiac endothelial cells [11]; however, it is also expressed on specific bone-marrow-derived cells, fibrocytes [17], leukocytes, vascular smooth muscle cells [18,19], and corneal epithelial and endothelial cells [20]. Another FB marker is fibroblast-specific protein 1, a filament-associated calcium-binding protein in FBs [21]; however, fibroblast-specific protein 1 has also been found to be expressed in leukocytes and a number of cancer cells [22]."
http://www.fibrogenesis.com/content/5/1/15
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