What is the smallest sized particle which can be studied through FACS?

Hi, I have a liposome of size 200-300 nm. If it will be labelled with fluorescence antibody, whether it can be studied through FACS? It is stable for FACS studies and we have LSR FORTESSA (BD) and...

01 February 2018 9,360 2 View

What are the percentages of Lymphocytes, B cells and T cells in murine splenocytes?

Hi, What are the percentages of Lymphocytes, B cells, T cells, CD4 cells, CD8 cells, Macrophages, DCs and NK cells in the mice spleen? what is the proportion of T and B cells in lymphocytes of...

31 December 2017 1,397 7 View

Why some people do live cell gating for Flow Cytometry study, even in the experiment involving intracellular staining?

For intracellular staining (such as for cytokines and transcription factors) in flow cytometry, we need to FIX & Permeablise the cells, prior the staining with fluorochrome-tagged antibodies....

02 March 2016 4,693 4 View

How much it is safe to use generic medicine?

Ethical Medicines are costly, but as and when the patent of a drug expires, any other company can composite the drug and cell it with much lower cost. Due to little variation of composition...

11 December 2015 7,183 1 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Which of the Covid-19 Vaccines would elicit the best immunoprotection and why?

I want to know which of the available Covid-19 Vaccines would elicit the best immunological response and why

02 March 2021 1,388 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View