Does anybody know that how can I evaluate IgG and A by the methods of "Indirect Sperm Mar Test with Sperm Mar Kit"?

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Regarding HPLC; Anyone could provide a detailed methods for simultaneous HPLC determination of GSH and GSSG in biological sample (e.g liver tissue)?

Dear colleagues, Hi Anyone could provide a detailed methods for simultaneous HPLC determination of GSH and GSSG in biological sample (e.g liver tissue). please indicate flow rate, sample...

31 December 2017 8,174 5 View

HI Dear Friends. in previouse study i evaluated CD3 for counting of LymT, at the moment I want to evaluate lymB. what kind of Cd do you suggest to me?

In previous study We evaluated CD3 for counting of LymT in Rats by flow-cytometry, at the moment I want to evaluate lym B., whats your For this CD?Which Cd is suitable? Thanks a lot, Mehdi

09 October 2015 1,498 1 View

Would someone have a valuable book, notebook and/or manuscript or anything else (in Persian or English) to improve article writing skills?

Hi Guys, would someone have a valuable book, notebook and/or manuscript or anythings else to improve article writting skills? I know that we have to read alots articles in our area, but i want to...

08 September 2015 10,156 6 View

Does crude extraction using NaOH and Tris work well with Fungi?

I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you

08 August 2024 4,733 2 View

What precautions should be taken while handling S. aureus enterotoxin Type B in the lab?

I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...

07 August 2024 6,034 3 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

Do some Staphylococcus aureus strains have in vitro antimycobacterial activity ?

Some Staphylococcus aureus strains Inhibit the growth of Mycobacteria in Mueller Hinton Agar medium containing 10% OADC. Do some Staphylococcus aureus strains have in vitro antimycobacterial activity?

29 July 2024 10,023 2 View

How to conduct a sensitivity power analysis for Kendall's Tau?

Is there a straightforward way to conduct a sensitivity power analysis for a Kendall's Tau correlation? I was considering using the sensitivity setting and "Correlation: point biserial model" test...

28 July 2024 6,133 8 View

Why my IgG antibody looks fragmented on non-reducing SDS-PAGE?

I am performing IgG purification and I have to show my results on SDS-PAGE. I use 10% tris glycine gel and prepare the samples under non reducing conditions. I am new to antibodies and therefore...

23 July 2024 6,664 6 View

What are the strategies to Enhance IgG-Producing Plasma Cells in mice for Monoclonal Antibody Development?

I have immunized BalB/C mice with a protein using the intradermal (ID) method with Complete Freund's Adjuvant (CFA) and Incomplete Freund's Adjuvant (IFA), following a 14-day interval and three...

22 July 2024 9,160 2 View

How can I calculate minimal sample size using G Power?

I am conducting a study aimed at exploring the correlation between introversion and social phobia among Lebanese adults. I need to calculate the minimal sample size using G Power, but I'm not sure...

19 July 2024 6,048 1 View

The reason for breeding Loxp mice with FLPe mice?

I am planning to use CD4 Cre mice crossed with Loxp mice. However, the laboratory from which I am trying to get the loxp sperm says they have "the product of the EMMA ko crossed with the FLPe to...

16 July 2024 7,475 1 View