Following CD4 T cell depletion, how long should i hang the anti-CD4 in the blood? If i want to do adoptive transfer of CD4 T cell, how long should I wait to have the least effect due to previously-injected anti-CD40?
I think it depends on the kind of preconditioning and the type of experiments you are doing. We regularly do HSC transplantation and we inject anti-CD8 the day before actual transplantation and anti-CD40 on the actual day a couple of hours before the transplantation, and it works well for us.