Problems with purification of IgG 1 Mab?

I have problems to purify an IgG1 Mab. How I can do it? The concentration in protein A pH6 is very low

07 August 2015 9,576 6 View

Standard curve shifting over time. How do I stabilise the ELISA?

24 February 2021 1,365 3 View

How do I prepare and store insect samples for JH measurements?

I need to preserve termite samples for future quantification of juvenile hormone in their bodies. Or extract the Juvenile Hormone and keep that samples stored for future quantification. I may need...

09 February 2021 1,995 1 View

Why cloning of gene into pCAMBIA1304 vector is unsuccessful?

I have tried to clone a specific gene into pCAMBIA1304 vector. But all the attempts failed. I used ligation kit from both Promega Corporation and Takara Bio. However, there was one time I used...

04 February 2021 525 1 View

What are the conflicts between EIA and environmental engineers and lawyers?

Environmental Impact Assessment (EIA) does not live up to the expectation of environmental lawyers and environmental engineers. In your own opinion, how can the EIA process therefore be made more...

03 February 2021 1,329 6 View

What Buffer should i use for T4 virus for electron microscopy ?

Hello, I currently have my T4 virus in HEPES buffer. Unfortunately, there are always crystals that are contaminating my solution (due to the buffer in which the Viruses are) when I observe them...

01 February 2021 400 3 View

How to prepare secondary antibody from lyophilized antibody?

So I'm new to lab research and learning Western Blots these days. I ordered my secondary antibody and it arrived in a powdered form. Here is the...

27 January 2021 5,402 3 View

Colorimteric and enzymatic method ?

First of all, thank you for your consideration about this question. I've been working on development of in-vitro diagnostic medical device as research engineer since 2013. Especially, my major is...

14 January 2021 3,885 4 View

Which syringe filters (e.g., PTFE, MCE, PVDF, CA, PES, etc.) are the best to filter TBS/PBS buffer (pH 7.5-8.5) and glycine buffer (pH 2-3) solutions?

I intend to purify antibodies from serum using the affinity procedure, and I need to filter the binding (TBS/PBS) and elution (Glycine) buffers before applying them to my solution.

06 January 2021 5,769 3 View

Any Custom Peptide Synthesis Companies In India? Custom Peptides Manufacturing Lab in India?

We are looking for many Custom Peptides in India, with good quality for our Bio-pharmaceutical research. Please suggest us best Custom Peptide Synthesis and Service Providers from India? Expecting...

31 December 2020 6,516 7 View

Greiner Bio or Corning Costar plates for ELISA?

09 December 2020 237 2 View