MInimisation before docking?

I am trying to do both docking and molecular dynamics simulation. Should I minimise the protein structure after adding amino acids far from active site before docking? I have taken a PDB structure...

02 November 2020 4,798 4 View

SENP1 treatment of CoImmunoprecipiteted proteins?

Dear Researcher, I am trying to imminoprecipitate (IP) Sumoylated protein. i am succesfully able to get it. now i have to carry out desumoylation of that immunoprecipiteted proteins in vitro. I...

27 February 2017 6,419 3 View

May I know somebody interested to collaborate in academic work about 'Adolescent SRH in Nepal (a quantitative study'?

27 January 2017 413 4 View

No title

10 January 2017 3,671 1 View

Can XRD curves for a polymer composite be normalized? If yes, then how?

I would like to normalize the intensity of different XRD curves, to be able to compare them.

14 December 2016 3,110 21 View

Can anyone suggest a simple calculation procedure to prepare any solution in terms of ppm ?

I am very confident and experienced enough in preparing the solutions up to ppm level when it is a SOLID, but I am confused in the case of LIQUIDS, say for example Hexadecane (hydrocarbon) has...

16 February 2015 9,202 18 View

Does use of a variable speed drive always ensure energy saving at reduced speed?

Vsd during running would result into some losses in terms of heat..my doubt is whether energy saving can be achieved at every range of reduced speed ( say @95%) considering those losses?

17 May 2014 6,855 4 View

Radiation from a porous region?

I am modelling porous medium combustion in FLUENT with a separate energy equation for the fluid and the solid zone. Since the temperature of the solid region is quite high (around 1200 K),...

11 February 2014 4,012 3 View

Do you know of a mesh based micro structure representation algorithm?

I wish to represent physical phase boundaries in a porous structure on a mesh. It seems to be quite simple to get a porous structure with the desired porosity by mere random allocation of solid...

22 January 2014 1,004 1 View

Which is the best company for ordering antibodies to immunophenotype mouse tissue by IHC ?

I am planning to immunophenotype mouse tissue that is infected with virus by Immunohistochemistry. I would like to know which company's antibodies works best for murine tissues? I have found a...

24 October 2013 5,262 25 View

What is the most Operating System (OS) which can be INSTALL on Tiny hard devices like Raspberry Pi (RPi) hardware ?

I'm targeting to deploy a mesh network and manually configure MANET routing protocols. I'm preparing scenarios, architectures, and hard devices needed to do that. Are there some step-by-step...

03 March 2021 1,931 5 View

Which of the Covid-19 Vaccines would elicit the best immunoprotection and why?

I want to know which of the available Covid-19 Vaccines would elicit the best immunological response and why

02 March 2021 1,388 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

How can I integrate a routing protocol in a tiny device (e.g. Raspberry Pi)?

Hi Community, I'm facing the issue of integration/compiling a new routing protocol in a WSN simulator. The final goal is to successfully add, configure this routing protocol in hardware devices...

01 March 2021 9,332 6 View

Anyone know of a good progesterone antibody?

I am looking for a good progesterone antibody to quantify levels of progesterone in zebrafish. Applications would be ELISA, and immunohistochemistry.

28 February 2021 5,929 2 View

How to compare 2 samples recruited with different sampling methods?

If I want to compare characteristics of 2 groups of patients, one group is recruited from a random sample of the general public while another group is from a sample with a fixed gender (male to...

26 February 2021 3,526 3 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

Experimental Design: How deal with pseudreplicates with immunological and clinical-chemical paramaeters?

Hello everybody, I am investigating the influence of a toxinon various clinical-chemical and haematological parameters in fish. I have three treatments (control, low dose, high dose) in my...

24 February 2021 1,243 1 View

Standard curve shifting over time. How do I stabilise the ELISA?

24 February 2021 1,365 3 View