Hi,
Can I use 37% formaldehyde (~10% methanol) to prepare 4% formaldehyde sol. in PBS for mice perfusion? How methanol can affect immunohistochemistry?
Hello,
Yes you can, the solution you are trying to prepare is a common PFA alternative called 10% Neutral Buffered Formalin.
You mix 1 part of 37-40% formaldehyde solution and 9 parts of PBS. There are many publications using this fixative, and it should work just fine.
Best of luck,
George
Thanks a lot!
Best regards,
Alex
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