What are the proper conditions to fix isolated mouse retina (fixative solution, time, temperature) before embedding into OCT prior to cryo-sectioning?
Hello Mohammed Hassan
Have you referred to the paper attached below? It may be helpful.
Article A quick protocol for the preparation of mouse retinal cryose...
Best.
You have 2 possibilities:
1. cut off the lense along the Ora serata. If the retina falls out of the eye cup transfer the tissue carefully in another vial containing 4 % PFA or 4% Formaldehyde. Duration of fixation depends of the sice of the retina, mouse rat retinae 3-5 days, bigger once like cat or human 1 week. For cryoprotection put the retina in 30% sucros . Leave it there until the retina lays on the bottom of the vial. Then embed it in OCT.
2. If the retina sticks in the eye cup, fix the eye cup completely with the retina as discribed above. Then peel off the retina out of the eye cup and transfer it into 30% sucrose for cryoprotection.
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